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首页> 外文期刊>Biotechnology and Bioengineering >Physiological evaluation of a new Chlorella sorokiniana isolate for its biomass production and lipid accumulation in photoautotrophic and heterotrophic cultures
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Physiological evaluation of a new Chlorella sorokiniana isolate for its biomass production and lipid accumulation in photoautotrophic and heterotrophic cultures

机译：一种新的小球藻小球藻在光养和异养培养中生物量产生和脂质积累的生理评估

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摘要

A novel green unicellular microalgal isolate from the freshwater of the Inner Mongolia Province of China and named as CCTCC M209220, grows between pH 6 and 11 and temperatures of 20-35°C with optimal conditions at pH 9 and 30°C. Morphological features and the phylogenetic analysis for the 18S rRNA gene reveal that the isolate is a Chlorella sorokiniana strain. A nitrogen source test reveals that this strain can grow well with nitrate and urea, but not ammonium. The strain can grow heterotrophically with glucose as the carbon source and accumulates lipid content as high as 56% (w/w) dry weight after 7 days in high glucose concentrations compared to 19% lipids achieved in 30 days of photoautotrophic culture. The relative neutral lipid content as a fraction of the total lipid is also much higher in heterotrophic culture as compared to photoautotrophic culture.
机译：一种来自中国内蒙古省淡水的新型绿色单细胞微藻分离物，命名为CCTCC M209220，可在pH 6至11和20-35°C的温度下生长，最佳条件是pH 9和30°C。 18S rRNA基因的形态学特征和系统发育分析表明，该分离物为小球藻小球藻菌株。氮源测试表明，该菌株可以与硝酸盐和尿素一起很好地生长，但不能与铵一起生长。该菌株可以在葡萄糖为碳源的情况下异养生长，并且在高葡萄糖浓度下7天后，脂质含量可累积高达56％（w / w）的干重，而在光合自养培养30天中达到19％的脂质。与光自养培养相比，异养培养中相对中性脂质含量（占总脂质的一部分）也要高得多。

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