首页> 外文期刊>American Journal of Physiology >Effect of changing vascular volume on measurement of protein reflection coefficient in ischemic lungs.
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Effect of changing vascular volume on measurement of protein reflection coefficient in ischemic lungs.

机译:血管体积变化对缺血性肺中蛋白质反射系数测量的影响。

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摘要

In ischemic organs, the protein reflection coefficient (sigma) can be estimated by measuring blood hematocrit (Hct) and protein after increasing static vascular pressure (P(v)). Our original equation for sigma (J Appl Physiol 73: 2616-2622, 1992) assumed a constant vascular volume during convective fluid flux (). In this study, we 1) quantified the rate of vascular volume change (dV/dt) still present in ischemic single ferret lungs after 20 min of P(v) = 30 Torr and 2) developed an equation for sigma that allowed a finite dV/dt. In 25 lungs, we estimated the dV/dt after 20 min at P(v) = 30 Torr by subtracting from the rate of lung weight gain (W(L)). The relationship between (0.15 +/- 0.02 ml/min) and W(L) (0.24 +/- 0.02 g/min) was significant (R = 0.66, P < 0.001), but the slope was <1 (0.41 +/- 0.10, P < 0.05). dV/dt (0.10 +/- 0.02 ml/min) was similar in magnitude to at 20 min. The modified equation for sigma revealed that a finite dV/dt caused the original sigma measurement to underestimate true sigma. A low sigma, high, high baseline Hct, and long filtration time enhanced the error. The error was small, however, and could be minimized by adjusting experimental parameters.
机译:在缺血性器官中,可以通过测量血液中的血细胞比容(Hct)和增加静态血管压力(P(v))后的蛋白质来估计蛋白质反射系数(sigma)。我们对sigma的原始方程式(J Appl Physiol 73:2616-2622,1992)假设对流流体通量()时血管体积恒定。在这项研究中,我们1)量化了20分钟的P(v)= 30 Torr后仍存在于缺血性单雪貂肺中的血管体积变化率(dV / dt),以及2)建立了允许有限dV的sigma方程/ dt。在25个肺中,我们通过减去肺增重率(W(L))来估算P(v)= 30 Torr时20分钟后的dV / dt。 (0.15 +/- 0.02 ml / min)和W(L)(0.24 +/- 0.02 g / min)之间的关系很明显(R = 0.66,P <0.001),但斜率<1(0.41 + / -0.10,P <0.05)。 dV / dt(0.10 +/- 0.02 ml / min)的大小与20分钟时相似。修改后的sigma方程表明,有限的dV / dt导致原始sigma测量值低估了真实的sigma。低sigma,高,高基线Hct和较长的过滤时间会增加误差。该误差很小,但是可以通过调整实验参数将其最小化。

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