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首页> 外文期刊>American Journal of Physiology >Neuronal nitric oxide synthase is expressed in principal cell of collecting duct.
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Neuronal nitric oxide synthase is expressed in principal cell of collecting duct.

机译：神经元一氧化氮合酶在集管的主细胞中表达。

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We used the RT-PCR technique and immunocytochemical methods to determine the expression of endothelial nitric oxide synthase (eNOS) or neuronal nitric oxide synthase (nNOS) in the cortical collecting duct (CCD) in rats on high-K+ diet. The microdissected CCDs of the rat kidney were lysed, and RT-PCR was carried out using rat nNOS and eNOS gene-specific primers. Southern analysis showed the presence of mRNA of nNOS but not eNOS in the CCD. The presence of nNOS in the CCD was further confirmed by light microscopy. We used the polyclonal nNOS antibody in immunocytochemical studies of the isolated CCD. We found that immunoreactivity to nNOS was present in the CCD and heterogeneous with positive and negative immunostaining. We performed the immunocytochemical studies in the split-open CCD and found that the immunoreactivity to nNOS was detected only in principal cells but not in intercalated cells. We conclude that nNOS is expressed in the rat CCD in rats on high-K+ diet. The presence of nNOS in the CCD is heterogeneous and mainly located in principal cells.
机译：我们使用RT-PCR技术和免疫细胞化学方法来确定高K +饮食的大鼠皮质收集管（CCD）中内皮型一氧化氮合酶（eNOS）或神经元一氧化氮合酶（nNOS）的表达。裂解大鼠肾脏的显微切割的CCD，并使用大鼠nNOS和eNOS基因特异性引物进行RT-PCR。 Southern分析表明，CCD中存在nNOS的mRNA而不是eNOS。通过光学显微镜进一步证实了CCD中nNOS的存在。我们在分离的CCD的免疫细胞化学研究中使用了多克隆nNOS抗体。我们发现CCD中存在对nNOS的免疫反应性，并且具有阳性和阴性免疫染色异质性。我们在开放式CCD中进行了免疫细胞化学研究，发现对nNOS的免疫反应仅在主细胞中检测到，而在插层细胞中未检测到。我们得出结论，在高K +饮食的大鼠CCD中，nNOS表达。 CCD中nNOS的存在是异质的，并且主要位于主要细胞中。

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