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首页> 外文期刊>American Journal of Physiology >Increased expression of ileal apical sodium-dependent bile acid transporter in postpartum rats.
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Increased expression of ileal apical sodium-dependent bile acid transporter in postpartum rats.

机译：产后大鼠回肠顶端钠依赖性胆汁酸转运蛋白的表达增加。

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摘要

The expression and activity of the apical ileal sodium-dependent bile acid transporter (asbt) was examined in the small intestine of control, pregnant, and lactating postpartum rats 2, 12, and 21 days after delivery. Western blot analysis of brush border membrane vesicles (BBMV) prepared from different regions of the small intestine demonstrated that expression of asbt was maximal in the most distal segments for all experimental groups, was not substantially affected in pregnant and 2-day postpartum rats, and was significantly increased in 12- and 21-day postpartum rats. Analysis of mRNA suggested that asbt protein was regulated at the posttranscriptional level in postpartum rats. Increased expression of asbt protein postpartum was maximal (approximately 2-fold) in the proximal region of the ileum, consistent with a 60% increase in taurocholate (TC) transport in BBMV from the proximal ileum in 14- to 21-day postpartum rats relative to control rats. Absorption of TC, determined from the intact proximal ileum using an intestinal loop model, demonstrated a 30% increase in TC uptake per unit weight of tissue in 14- to 21-day postpartum rats relative to control rats. Together with the marked increase in intestinal mass observed at peak lactation, these data indicate a significant increase in asbt-mediated reclamation of bile acids in the intestine of lactating rats.
机译：在分娩后第2、12和21天，在对照组，妊娠和哺乳期的产后大鼠的小肠中检查了顶端回肠钠依赖性胆汁酸转运蛋白（asbt）的表达和活性。从小肠不同区域制备的刷状缘膜囊泡（BBMV）的Western印迹分析表明，对于所有实验组，最远端段的asbt表达最大，在怀孕和产后2天的大鼠中基本上没有受到影响，并且在产后12天和21天的大鼠中，其显着增加。对mRNA的分析表明，产后大鼠的asbt蛋白在转录后水平受到调节。回肠近端区域产后asbt蛋白表达的增加最大（约2倍），与产后14到21天相对于回肠的BBMV中牛磺胆酸盐（TC）转运从回肠近端增加60％有关来控制老鼠。使用完整肠回肠模型从完整的近端回肠确定的TC吸收量表明，相对于对照大鼠，产后14至21天大鼠的单位重量组织中TC吸收量增加了30％。这些数据与泌乳高峰期观察到的肠道质量显着增加一起，表明哺乳期大鼠肠道中asbt介导的胆汁酸回收率显着增加。

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