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首页> 外文期刊>American Journal of Physiology >A novel form of cellular communication among thymic epithelial cells: intercellular calcium wave propagation.
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A novel form of cellular communication among thymic epithelial cells: intercellular calcium wave propagation.

机译：胸腺上皮细胞之间一种新型的细胞通讯方式：细胞间钙波传播。

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We here describe intercellular calcium waves as a novel form of cellular communication among thymic epithelial cells. We first characterized the mechanical induction of intercellular calcium waves in different thymic epithelial cell preparations: cortical 1-4C18 and medullary 3-10 thymic epithelial cell lines and primary cultures of thymic "nurse" cells. All thymic epithelial preparations responded with intercellular calcium wave propagation after mechanical stimulation. In general, the propagation efficacy of intercellular calcium waves in these cells was high, reaching 80-100% of the cells within a given confocal microscopic field, with a mean velocity of 6-10 microm/s and mean amplitude of 1.4- to 1.7-fold the basal calcium level. As evaluated by heptanol and suramin treatment, our results suggest the participation of both gap junctions and P2 receptors in the propagation of intercellular calcium waves in thymic nurse cells and the more prominent participation of gap junctions in thymic epithelial cell lines. Finally, in cocultures, the transmission of intercellular calcium wave was not observed between the mechanically stimulated thymic epithelial cell and adherent thymocytes, suggesting that intercellular calcium wave propagation is limited to thymic epithelial cells and does not affect the neighboring thymocytes. In conclusion, these data describe for the first time intercellular calcium waves in thymic epithelial cells and the participation of both gap junctions and P2 receptors in their propagation.
机译：我们在这里将细胞间钙波描述为胸腺上皮细胞之间细胞通讯的一种新型形式。我们首先表征了在不同胸腺上皮细胞制备物中的细胞间钙波的机械诱导：皮质1-4C18和延髓3-10胸腺上皮细胞系以及胸腺“护士”细胞的原代培养。机械刺激后，所有胸腺上皮制剂均响应细胞间钙波的传播。通常，在这些细胞中细胞间钙波的传播效率很高，在给定的共聚焦显微镜视野内达到细胞的80-100％，平均速度为6-10 microm / s，平均振幅为1.4-1.7倍基础钙水平。通过庚醇和苏拉明治疗评估，我们的结果表明，间隙连接和P2受体均参与胸腺护士细胞中细胞间钙波的传播，并且间隙连接在胸腺上皮细胞系中的作用更为突出。最后，在共培养物中，在机械刺激的胸腺上皮细胞和粘附的胸腺细胞之间未观察到细胞间钙波的传播，这表明细胞间钙波的传播仅限于胸腺上皮细胞，并且不影响邻近的胸腺细胞。总之，这些数据首次描述了胸腺上皮细胞中的细胞间钙波以及间隙连接和P2受体在其增殖中的参与。

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