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首页> 外文期刊>American Journal of Physiology >Isolated mouse pancreatic beta-cells show cell-specific temporal response pattern.
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Isolated mouse pancreatic beta-cells show cell-specific temporal response pattern.

机译：分离的小鼠胰腺β细胞显示特定于细胞的时间反应模式。

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摘要

The length of the silent lag time before elevation of the cytosolic free Ca2+ concentration ([Ca2+]i) differs between individual pancreatic beta-cells. One important question is whether these differences reflect a random phenomenon or whether the length of lag time is inherent in the individual beta-cell. We compared the lag times, initial dips, and initial peak heights for [Ca2+]i from two consecutive glucose stimulations (with either 10 or 20 mM glucose) in individual ob/ob mouse beta-cells with the fura 2 technique in a microfluorimetric system. There was a strong correlation between the lengths of the lag times in each beta-cell (10 mM glucose: r = 0.94, P < 0.001; 20 mM glucose: r = 0.96, P < 0.001) as well as between the initial dips in [Ca2+]i (10 mM glucose: r = 0.93, P < 0.001; 20 mM glucose: r = 0.79, P < 0.001) and between the initial peak heights (10 mM glucose: r = 0.51, P < 0.01; 20 mM glucose: r = 0.77, P < 0.001). These data provide evidence that the response pattern, including both thelength of the lag time and the dynamics of the subsequent [Ca2+]i, is specific for the individual beta-cell.
机译：单个胰岛β细胞之间的游离胞质Ca2 +浓度（[Ca2 +] i）升高之前，沉默滞后时间的长度有所不同。一个重要的问题是这些差异是否反映出一种随机现象，或者滞后时间的长短是否是单个β细胞固有的。我们用呋喃2技术在微荧光系统中比较了单个ob / ob小鼠β细胞中两次连续葡萄糖刺激（含10或20 mM葡萄糖）的[Ca2 +] i的滞后时间，初始下陷和初始峰高。 。每个β细胞的滞后时间长度之间有很强的相关性（10 mM葡萄糖：r = 0.94，P <0.001; 20 mM葡萄糖：r = 0.96，P <0.001）以及初始浸入之间[Ca2 +] i（10 mM葡萄糖：r = 0.93，P <0.001; 20 mM葡萄糖：r = 0.79，P <0.001）和初始峰高之间（10 mM葡萄糖：r = 0.51，P <0.01; 20 mM葡萄糖：r = 0.77，P <0.001）。这些数据提供了包括滞后时间长度和随后的[Ca2 +] i动力学在内的响应模式对于单个β细胞的特异性。

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