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首页> 外文期刊>American Journal of Physiology >Cytokine gene expression in human skeletal muscle during concentric contraction: evidence that IL-8, like IL-6, is influenced by glycogen availability.
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Cytokine gene expression in human skeletal muscle during concentric contraction: evidence that IL-8, like IL-6, is influenced by glycogen availability.

机译：同心收缩过程中人骨骼肌中细胞因子基因的表达：证据表明IL-8与IL-6一样受糖原利用率的影响。

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摘要

To determine the expression and induction of cytokines in human skeletal muscle during concentric contractions, eight males performed 60 min of bicycle exercise, with either a normal (Con) or reduced (Lo Gly) preexercise intramuscular glycogen content. Muscle biopsy samples were obtained before and after exercise and analyzed for glycogen and the mRNA expression of 13 cytokines. Resting muscle glycogen was higher (P < 0.05) in Con compared with Lo Gly and was reduced (P < 0.05) to 102 +/- 32 vs. 17 +/- 5 mmol U glycosyl/kg dry mass for Con and Lo Gly, respectively. We detected mRNA levels in human skeletal muscle for five cytokines, namely interleukin (IL)-1 beta, IL-6, IL-8, IL-15, and tumor necrosis factor-alpha. However, muscle contraction increased (P < 0.05) the mRNA expression of IL-6 and IL-8 alone. In addition, the fold change for both IL-8 and IL-6 was markedly higher (P < 0.05) in Lo Gly compared with Con. Given these results, we analyzed venous blood samples, obtained before and during exercise, for IL-6 and IL-8. Plasma IL-6 was not different at rest, and although the circulating concentration of this cytokine increased (P < 0.05) it increased to a greater extent (P < 0.05) throughout exercise in Lo Gly. In contrast, plasma IL-8 was not affected by exercise or treatment. These data demonstrate that cytokines are not ubiquitously expressed in skeletal muscle and that only IL-6 and IL-8 mRNA are increased during contraction of this mode and duration. Furthermore, the mRNA abundance of IL-6 and IL-8 appears to be influenced by glycogen availability in the contracting muscle.
机译：为了确定同心收缩过程中人骨骼肌中细胞因子的表达和诱导，八名男性进行了60分钟的自行车运动，运动前肌内糖原含量正常（Con）或降低（Lo Gly）。运动前后进行肌肉活检，并分析糖原和13种细胞因子的mRNA表达。 Con的静息肌糖原高于Lo Gly（P <0.05），而Con和Lo Gly的静糖肌糖原减少至（102 <32）vs. 17 +/- 5 mmol U糖基/ kg干质量，分别。我们检测到人类骨骼肌中5种细胞因子的mRNA水平，即白介素（IL）-1 beta，IL-6，IL-8，IL-15和肿瘤坏死因子-α。但是，肌肉收缩增加了IL-6和IL-8单独的mRNA表达（P <0.05）。另外，与Con相比，Lo Gly中IL-8和IL-6的倍数变化均显着更高（P <0.05）。鉴于这些结果，我们分析了运动前和运动中获得的静脉血样本中的IL-6和IL-8。血浆IL-6静止时无差异，尽管这种细胞因子的循环浓度增加（P <0.05），但在Lo Gly的整个运动过程中，其增加程度更大（P <0.05）。相反，血浆IL-8不受运动或治疗的影响。这些数据证明在骨骼肌中不普遍表达细胞因子，并且在该模式和持续时间的收缩过程中仅IL-6和IL-8 mRNA升高。此外，IL-6和IL-8的mRNA丰度似乎受到收缩肌肉中糖原利用率的影响。

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