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首页> 外文期刊>American Journal of Physiology >Functional overlap of IP(3)- and cADP-ribose-sensitive calcium stores in guinea pig myenteric neurons.
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Functional overlap of IP(3)- and cADP-ribose-sensitive calcium stores in guinea pig myenteric neurons.

机译：IP（3）和cADP核糖敏感钙存储在豚鼠肌间神经元中的功能重叠。

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摘要

In myenteric neurons two different receptor subtypes govern the intracellular Ca(2+) stores: the inositol 1,4,5-trisphosphate (IP(3)) receptor (IP(3)R) and the ryanodine receptor (RyR). Their degree of functional overlap was determined by examining Ca(2+) release in these cells through both superfusion techniques and intracellular microinjection. Microinjection of IP(3) (50 microM) and cADP-ribose (cADPr, 50 microM), specific ligands for the IP(3)R and RyR, respectively, demonstrated mobilization of intracellular Ca(2+) stores. Perfusion with cinnarizine (50 microM) or dantrolene (10 microM), antagonists of the IP(3)R and RyR, respectively, eliminated the Ca(2+) response to microinjected IP(3) and cADPr. Superfusion of the neurons with 100 microM ATP, an IP(3)-mediated Ca(2+)-mobilizing agonist, caused intracellular Ca(2+) increments, which were antagonized by cinnarizine, and the RyR antagonists dantrolene, procaine (5 mM), and ryanodine (1 microM). Caffeine (10 mM) was applied repetitively in Ca(2+)-free conditions to deplete RyR-sensitive stores; subsequent perfusion with ATP demonstrated a Ca(2+) response. Conversely, caffeine caused a Ca(2+) response after repetitive ATP exposures. The internal Ca(2+) stores of myenteric neurons are governed by two receptor subtypes, IP(3)R and RyR, which share partial functional overlap.
机译：在肌层神经元中，两种不同的受体亚型控制着细胞内Ca（2+）的储存：肌醇1,4,5-三磷酸（IP（3））受体（IP（3）R）和ryanodine受体（RyR）。他们的功能重叠的程度通过通过融合技术和细胞内显微注射检查这些细胞中的Ca（2+）释放来确定。 IP（3）（50 microM）和cADP-核糖（cADPr，50 microM），IP（3）R和RyR的特定配体的显微注射分别证明了细胞内Ca（2+）存储的动员。分别与IP（3）R和RyR的拮抗剂肉桂那嗪（50 microM）或dantrolene（10 microM）灌注，消除了Ca（2+）对微注射IP（3）和cADPr的反应。神经元与100 microM ATP，IP（3）介导的Ca（2+）激动剂的超融合，引起细胞内Ca（2+）的增加，并被肉桂那嗪和RyR拮抗剂丹特罗，普鲁卡因（5 mM）拮抗）和ryanodine（1 microM）。咖啡因（10毫米）重复应用在无Ca（2+）的条件下，以耗尽RyR敏感的存储区;随后与ATP灌注显示Ca（2+）反应。相反，咖啡因重复ATP暴露后引起Ca（2+）反应。肌层神经元的内部Ca（2+）存储受两个受体亚型IP（3）R和RyR支配，它们共享部分功能重叠。

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