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首页> 外文期刊>American journal of rhinology >Inflammatory pathway gene expression in chronic rhinosinusitis.
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Inflammatory pathway gene expression in chronic rhinosinusitis.

机译：慢性鼻-鼻窦炎的炎症途径基因表达。

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BACKGROUND: The main objective in this preliminary experiment was to compare gene expression in the sinus mucosa of patients with chronic hyperplastic rhinosinusitis (CRS) against normal subjects, using gene microarray technology. The specific aim was to examine alterations in inflammatory mediator expression in patients with CRS. We performed a prospective experimental study. METHODS: Total RNA samples were obtained from the sinus mucosa biopsies of 14 patients with chronic hyperplastic sinusitis and 4 normal controls, using the Affymetrix recommended protocol. The data for 22,000 genes on the GeneChip U133A were generated from 18 hybridizations. Affymetrix GeneChip 5.0 was used as the image acquisition software for the U133A chips. Data normalization, log transformation, statistical analysis, and pattern study were performed with GeneSpring software. Comparison between patients with CRS and normal controls were performed using the Welch t-test, with log transformed data. RESULTS: There were a total of 1283 genes scored as differentially expressed between groups. The value of p, the probability of a false positive, was set to <0.05. Hierarchical clustering was applied to study coexpression patterns of the 1283 significant genes. The inflammatory pathway was overlaid with the differential expressed gene list. Four genes involved in the inflammatory pathway, interleukin (IL)-6, IL-12A, IL-13, and tumor necrosis factor (TNF) alpha (2) were consistently overexpressed in patients with hyperplastic CRS. CONCLUSION: There is overexpression of four major genes of the inflammatory pathway (IL-6, IL-12A, IL-13, and TNF-alpha (2)) in patients with CRS compared with the normal population. Defining gene expression profiles may help elucidate new key factors in the pathogenesis of CRS and perhaps aid in the development of new therapeutic modalities.
机译：背景：这项初步实验的主要目的是使用基因芯片技术比较慢性增生性鼻－鼻窦炎（CRS）患者与正常受试者的鼻窦黏膜中的基因表达。具体目的是检查CRS患者炎症介质表达的变化。我们进行了一项前瞻性实验研究。方法：采用Affymetrix推荐方案，从14例慢性增生性鼻窦炎患者和4例正常对照的鼻窦黏膜活检组织中提取总RNA样品。通过18次杂交获得了GeneChip U133A上22,000个基因的数据。 Affymetrix GeneChip 5.0被用作U133A芯片的图像采集软件。数据归一化，对数转换，统计分析和模式研究均使用GeneSpring软件进行。使用Welch t检验，对数转换后的数据，对CRS患者和正常对照组进行比较。结果：总共有1283个基因在两组之间有差异表达。 p的值为假阳性的概率设置为<0.05。层次聚类用于研究1283个重要基因的共表达模式。炎性途径被差异表达基因列表所覆盖。在增生性CRS患者中，与炎症途径有关的四个基因，白介素（IL）-6，IL-12A，IL-13和肿瘤坏死因子（TNF）α（2）始终过量表达。结论：与正常人群相比，CRS患者的炎症途径的四个主要基因（IL-6，IL-12A，IL-13和TNF-alpha（2））过表达。定义基因表达谱可能有助于阐明CRS发病机理中的新关键因素，并可能有助于开发新的治疗方式。

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