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首页> 外文期刊>American journal of rhinology >The expression of MUC5AC and MUC5B mucin genes in the mucosa of chronic rhinosinusitis and nasal polyposis.
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The expression of MUC5AC and MUC5B mucin genes in the mucosa of chronic rhinosinusitis and nasal polyposis.

机译：MUC5AC和MUC5B粘蛋白基因在慢性鼻-鼻窦炎和鼻息肉的粘膜中的表达。

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BACKGROUND: The aim of this study was to investigate the expression of MUC5AC and MUC5B messenger RNAs (mRNAs) and localization of these proteins in human sinus mucosa of chronic rhinosinusitis (CRS) and CRS with nasal polyposis (CRS/NP). METHODS: Maxillary sinus ostia mucosa was harvested from patients undergoing endoscopic sinus surgery for CRS, CRS/NP, and non-CRS pathologies (control). Then, sinus mucosa was analyzed using reverse-transcription polymerase chain reaction to detect mRNA of MUC5AC and MUC5B. Hematoxylin and eosin staining and immunofluorescent staining were used to localize MUC5AC and MUC5B proteins in the sinus mucosa. RESULTS: mRNAs of MUC5AC and MUC5B in the sinus mucosa of CRS and CRS/NP were significantly increased compared with that in normal sinus mucosa (p < 0.01), and no significant difference was found between the mucosa of CRS and that of CRS/NP (p > 0.05). MUC5AC protein was expressed mainly in the goblet cells, and MUC5B expression was located in the submucosal glands cells and the epithelia of sinus mucosa. ARPC in staining of MUC5AC and MUC5B were found no different between the CRS group and the CRS/NP group (p > 0.05), whereas they were significantly lower in the normal group compared with the other two groups, respectively (p < 0.05). CONCLUSION: This study showed that MUC5AC and MUC5B mucin genes were up-regulated in sinus mucosa of CRS and CRS/NP. MUC5AC and MUC5B may play an important role in the pathogenesis of CRS and NP.
机译：背景：本研究的目的是研究MUC5AC和MUC5B信使RNA（mRNA）的表达以及这些蛋白在慢性鼻鼻窦炎（CRS）和鼻息肉病（CRS / NP）的CRS人鼻黏膜中的定位。方法：从接受内窥镜鼻窦手术的CRS，CRS / NP和非CRS病变（对照）患者中收集上颌窦口黏膜。然后，使用逆转录聚合酶链反应分析窦粘膜，以检测MUC5AC和MUC5B的mRNA。使用苏木精和曙红染色以及免疫荧光染色在鼻黏膜中定位MUC5AC和MUC5B蛋白。结果：与正常鼻窦黏膜相比，CRS和CRS / NP的鼻窦黏膜中MUC5AC和MUC5B的mRNA均显着增加（p <0.01），CRS黏膜与CRS / NP黏膜之间无明显差异。 （p> 0.05）。 MUC5AC蛋白主要在杯状细胞中表达，MUC5B表达位于黏膜下腺细胞和窦黏膜上皮中。 CRS组和CRS / NP组之间MUC5AC和MUC5B染色的ARPC均无差异（p> 0.05），而正常组的ARPC显着低于其他两组（p <0.05）。结论：本研究表明MUC5AC和MUC5B粘蛋白基因在CRS和CRS / NP的鼻窦黏膜中上调。 MUC5AC和MUC5B可能在CRS和NP的发病机理中起重要作用。

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