Library screening for D-amino-acid oxidase gene:Application of real-time PCR

R.Konno; A.Niwa

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Library screening for D-amino-acid oxidase gene:Application of real-time PCR

机译：D-氨基酸氧化酶基因的文库筛选：实时PCR的应用

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Quantitative real-time PCR shows the quantity in addition to the presence of the target sequence.This property seemed very useful for library screening.Then,real-time PCR was employed to screen for A phages carrying D-amino-acid oxidase gene from mouse genomic library.Using stepwise dilution screening combined with real-time PCR,positive phages were isolated in a short time.

机译：实时荧光定量PCR不仅显示了靶序列的存在，而且还显示了该数量。这一特性对于文库筛选非常有用。然后，采用实时PCR筛选小鼠D携带D-氨基酸氧化酶基因的噬菌体基因组文库。采用逐步稀释筛选与实时荧光定量PCR结合，可在短时间内分离出阳性噬菌体。

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《Amino acids》 |2004年第2期|共3页
作者
R.Konno; A.Niwa;
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原文格式 PDF
正文语种 eng
中图分类生物化学;
关键词
Real; time PCR; D; Amino; acid oxidase; Library; Screening; Mouse;

机译：实时PCR;D;氨基酸;酸氧化酶;文库;筛选;小鼠;

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1. Library screening for D-amino-acid oxidase gene:Application of real-time PCR [J] . R.Konno, A.Niwa Amino acids . 2004,第2期

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Library screening for D-amino-acid oxidase gene:Application of real-time PCR

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