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首页> 外文期刊>Anaerobe >The genus Prevotella in cystic fibrosis airways.
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The genus Prevotella in cystic fibrosis airways.

机译：囊性纤维化气道中的普雷沃氏菌属。

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Airway disease resulting from chronic bacterial colonization and consequential inflammation is the leading cause of morbidity and mortality in patients with Cystic Fibrosis (CF). Although traditionally considered to be due to only a few pathogens, recent re-examination of CF airway microbiology has revealed that polymicrobial communities that include many obligate anaerobes colonize lower airways. The purpose of this study was to examine Prevotella species in CF airways by quantitative culture and phenotypic characterization. Expectorated sputum was transferred to an anaerobic environment immediately following collection and examined by quantitative microbiology using a variety of culture media. Isolates were identified as facultative or obligate anaerobes and the later group was identified by 16S rRNA sequencing. Prevotella spp. represented the majority of isolates. Twelve different species of Prevotella were recovered from 16 patients with three species representing 65% of isolates. Multiple Prevotella species were often isolated from the same sputum sample. These isolates were biochemically characterized using Rapid ID 32A kits (BioMerieux), and for their ability to produce autoinducer-2 and beta-lactamases. Considerable phenotypic variability between isolates of the same species was observed. The quantity and composition of Prevotella species within a patients' airway microbiome varied over time. Our results suggest that the diversity and dynamics of Prevotella in CF airways may contribute to airway disease.
机译：由慢性细菌定植和继发性炎症引起的气道疾病是囊性纤维化（CF）患者发病和死亡的主要原因。尽管传统上认为仅由少数病原体引起，但最近对CF气道微生物学的重新检查显示，包括许多专性厌氧菌的多菌种群落在下气道定居。这项研究的目的是通过定量培养和表型表征来检查CF气道中的普氏杆菌物种。收集痰液后立即将其转移到厌氧环境中，并使用多种培养基通过定量微生物学检查。分离株被鉴定为兼性或专性厌氧菌，后一组通过16S rRNA测序鉴定。普氏杆菌属代表了大多数分离株。从16名患者中回收了12种不同的Prevotella，其中3种代表65％的分离株。通常从同一痰液样本中分离出多种Prevotella菌种。这些分离物使用Rapid ID 32A试剂盒（BioMerieux）进行生化鉴定，并具有产生autoinducer-2和β-内酰胺酶的能力。在相同物种的分离株之间观察到明显的表型变异性。患者气道微生物组中普雷沃氏菌物种的数量和组成随时间变化。我们的研究结果表明，CF气道中普雷沃氏菌的多样性和动态可能导致气道疾病。

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