Structures of the Catalytic Domain of Bacterial Primase DnaG in Complexes with DNA Provide Insight into Key Priming Events

Caixia Hou; Tapan Biswas; Oleg V. Tsodikov

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Structures of the Catalytic Domain of Bacterial Primase DnaG in Complexes with DNA Provide Insight into Key Priming Events

机译：用DNA复合物中细菌预碱基DNAG的催化结构域的结构提供了对关键引发事件的洞察力

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Bacterial primase DnaG is an essential nucleic acid polymerase that generates primers for replication of chromosomal DNA. The mechanism of DnaG remains unclear due to the paucity of structural information on DnaG in complexes with other replisome components. Here we report the first crystal structures of noncovalent DnaG–DNA complexes, obtained with the RNA polymerase domain of Mycobacterium tuberculosis DnaG and various DNA ligands. One structure, obtained with ds DNA, reveals interactions with DnaG as it slides on ds DNA and suggests how DnaG binds template for primer synthesis. In another structure, DNA in the active site of DnaG mimics the primer, providing insight into mechanisms for the nucleotide transfer and DNA translocation. In conjunction with the recent cryo-EM structure of the bacteriophage T7 replisome, this study yields a model for primer elongation and hand-off to DNA polymerase.

机译：细菌预级酶DNAG是一种基本核酸聚合酶，用于产生用于复制染色体DNA的引物。由于与其他重复组分的复合物中的DNAG的结构信息缺乏，DNAG的机制仍然不明确。在这里，我们报告了使用C结核分枝杆菌的RNA聚合结构域和各种DNA配体获得的非共价DNAG-DNA复合物的第一晶体结构。用DS DNA获得一种结构，揭示了与DNAG的相互作用，因为它在DS DNA上滑动并表明DNAG如何结合引物合成的模板。在另一种结构中，DNAG活性位点中的DNA模仿底漆，从而深入了解核苷酸转移和DNA易位的机制。结合最近的噬菌体T7替换物的Cryo-EM结构，该研究产生了底漆伸长和递送至DNA聚合酶的模型。

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《Biochemistry》 |2018年第14期|共10页
作者
Caixia Hou; Tapan Biswas; Oleg V. Tsodikov;
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Department of Pharmaceutical Sciences College of Pharmacy University of Kentucky Lexington Kentucky 40536 United States;

Department of Chemistry and Biochemistry University of California San Diego La Jolla California 92093 United States;

Department of Pharmaceutical Sciences College of Pharmacy University of Kentucky Lexington Kentucky 40536 United States;

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正文语种 eng
中图分类生物化学;
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1. 卤虫中编码DM结构域的DNA序列的克隆和初步研究(简报) [J] . 曾辉, 宋文芹, 陈瑞阳分子细胞生物学报（英文版） . 2004,第005期
2. Structures of the Catalytic Domain of Bacterial Primase DnaG in Complexes with DNA Provide Insight into Key Priming Events [J] . Caixia Hou, Tapan Biswas, Oleg V. Tsodikov Biochemistry . 2018,第14期

机译：用DNA复合物中细菌预碱基DNAG的催化结构域的结构提供了对关键引发事件的洞察力
3. Toprim--a conserved catalytic domain in type IA and II topoisomerases, DnaG-type primases, OLD family nucleases and RecR proteins. [J] . Aravind L, Leipe DD, Koonin EV Nucleic Acids Research . 1998,第18期

机译：Toprim-IA和II型拓扑异构酶，DnaG型启动酶，OLD家族核酸酶和RecR蛋白中的保守催化结构域。
4. Toprim—a conserved catalytic domain in type IA and II topoisomerases, DnaG-type primases, OLD family nucleases and RecR proteins [J] . Detlef D. Leipe, Eugene V. Koonin, L. Aravind Nucleic acids research . 1998,第18期

机译：Toprim-IA和II型拓扑异构酶，DnaG型启动酶，OLD家族核酸酶和RecR蛋白中的保守催化结构域
5. Support Vector Machine for Prediction of DNA-Binding Domains in Protein-DNA Complexes [C] . Jiansheng Wu, Hongtao Wu, Hongde Liu, International Conference on Life System Modeling and Simulation(LSMS 2007); 20070914-17; Shanghai(CN) . 2007

机译：支持向量机，预测蛋白质-DNA复合物中的DNA结合域
6. Part I: Structural and functional insight into the type A CBFbeta-SMMHC protein and acute myeloid leukemia (AML). Part II: Solution structure of the HDGF PWWP domain and implications of its function as a DNA binding domain. [D] . Lukasik, Stephen Michael. 2006

机译：第一部分：对A型CBFbeta-SMMHC蛋白和急性髓细胞性白血病（AML）的结构和功能洞察。第二部分：HDGF PWWP域的溶液结构及其作为DNA结合域的功能的含义。
7. Insights into eukaryotic DNA priming from the structure and functional interactions of the 4Fe-4S cluster domain of human DNA primase [O] . Sivaraja Vaithiyalingam, Eric M. Warren, Brandt F. Eichman, 2010

机译：从人类DNA引发酶的4Fe-4S簇结构域的结构和功能相互作用洞察真核DNA引发
8. Insights into eukaryotic DNA priming from the structure and functional interactions of the 4Fe-4S cluster domain of human DNA primase [O] . Vaithiyalingam, Sivaraja, Warren, Eric M., Eichman, Brandt F., 2010

机译：从人类DNA引发酶的4Fe-4S簇结构域的结构和功能相互作用洞察真核DNA引发

Structures of the Catalytic Domain of Bacterial Primase DnaG in Complexes with DNA Provide Insight into Key Priming Events

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