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首页> 外文期刊>Acta Horticulturae >Seed and protocorm development by in vitro ovary culture at the fertilization phase in Cymbidium
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Seed and protocorm development by in vitro ovary culture at the fertilization phase in Cymbidium

机译：在聚胞菌施肥期的体外卵巢培养种子和质子素

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In Cymbidium, the period from pollination to fertilization is about 3 months and the period from pollination to mature seed is about 10 months. It is difficult to observe sequential changes in fertilization and seed development because sufficient developing ovaries are needed. Often, the fruit abscises, and several ovaries are lost. Because the low viability of the pollinia was considered one of the causal factors of the abscission of the ovaries, mixed pollination of Cymbidium eburneum with the pollinia of three other species, C.floribundum, C. devonianum, and C. eburneum, was performed to produce the required number of ovaries. Pollinated pistils were collected every two weeks, sterilized, and sliced off horizontally. The column side was placed into fixing solution and the basal side was placed on 0.3% gellan-gum solidified MS medium supplemented with 5% sucrose, 10 mg L1 picloram and 1 mg L1 benzyl aminopurine. The initial developing ovules were observed in ovaries collected 4 weeks after pollination. In the ovaries collected 6 weeks after pollination, a pollen tube was observed near the ovule. The number of developing ovules and pollen tubes increased over the weeks. Nine months after pollination, the cultured ovaries were browning and splitting, and seeds were observed. Surviving seeds from the cultured ovaries started 7 weeks after pollination were found by FDA staining, and a few protocorms were obtained from the ovary culture started 6 weeks after pollination. The number of protocorms increased over the weeks. When C. lowianum were used as seed parents, protocorms were found from the ovary cultures started 10 weeks after pollination. These results suggest that the timing of ovule development and fertilization might not synchronize in thesame ovary. Moreover, fertilized ovules might continue to develop into seeds in these ovary cultures.
机译：在聚荷尼亚植物中，授粉对施肥的时间约为3个月，授粉对成熟种子的时间约为10个月。由于需要足够的发展卵巢，难以观察施肥和种子发展的顺序变化。通常，果实脓肿和几个卵巢都丢失了。由于人灵管的低可行性被认为是卵巢脱落的因果因素之一，但对三种其他物种的花球菌胞质的百合胞胎的混合授粉，C.Floribundum，C. devonianum和C.Eburneum进行了产生所需数量的卵巢。每两周收集授粉的雌蕊，灭菌，水平切片。将柱侧置于固定溶液中，将基底侧置于0.3％Gellan-Gum固化的MS培养基上，其补充有5％蔗糖，10mg L1糠醛和1mg L1苄基氨基嘌呤。在授粉后4周收集的卵巢中观察到初始显影胚珠。在授粉后6周收集的卵巢中，在胚珠附近观察到花粉管。显影胚珠和花粉管的数量在几周内增加。授粉后九个月，培养的卵巢是褐变和分裂，并且观察种子。通过FDA染色发现授粉7周后，从培养的卵巢中存活的种子，从授粉后6周开始，从卵巢培养物中获得一些质子染色。周数增加了质子。当使用C.LowInum被用作种子父母时，从溶液10周后开始的卵巢培养物中发现质子染色。这些结果表明，胚珠发育和施肥的时间可能不会在卵巢中同步。此外，受精卵可能在这些卵巢培养物中继续发展成种子。

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