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首页> 外文期刊>Acta Horticulturae >Characterization of strawberry cultivars by SSR and CAPS markers
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Characterization of strawberry cultivars by SSR and CAPS markers

机译:SSR和CAPS标记的草莓品种表征

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摘要

This study was performed to reveal the value of genetic relationship among 34 Turkish and foreign strawberry cultivars and genotypes using CAPS and SSR DNA molecular markers. Seventeen primer-enzyme combinations in CAPS and 12 SSR primer pairs in SSRanalysis were used. In CAPS analysis, 60 bands were produced and 52 of them were polymorphic. In SSR analysis, 150 alleles were generated and 145 of them were polymorphic. In the analysis of combined CAPS and SSR data, genetic similarity ranged between 0.59 and 0.91. 'Call Giant-2' and 'Call Giant-3' (0.91) were the closest cultivars, while Genotype 8 and Call Giant-4 were the most distant genotypes. As a result, SSR was better than CAPS from the point of view of polymorphism rates and polymorphism information contents, whereas CAPS was better than SSR from the point of view of resolving power values, in the characterization of strawberry cultivars and genotypes. The result of this study may allow us to design a cultivar breeding program in strawberry.
机译:本研究进行了使用帽和SSR DNA分子标记的34种土耳其和外国草莓品种和基因型之间的遗传关系的价值。使用17种引物 - 酶组合和在Ssranalysics中的12SSR引物对中的组合。在CAPS分析中,产生60个条带,其中52个是多态的。在SSR分析中,产生150个等位基因,其中145个是多态的。在分析组合帽和SSR数据的情况下,遗传相似性范围在0.59和0.91之间。 “呼叫巨头-2”和“呼叫巨头-3”(0.91)是最近的品种,而基因型8和呼叫巨头-4是最远处的基因型。结果,从多态性速率和多态性信息内容的角度来看,SSR优于盖子,而帽子从解决功率值的特征在于草莓品种和基因型的表征中,帽子比SSR更好。本研究的结果可能让我们在草莓中设计一种品种育种程序。

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