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首页> 外文期刊>American Journal of Physiology >Novel insights of the gastric gland organization revealed by chief cell specific expression of moesin
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Novel insights of the gastric gland organization revealed by chief cell specific expression of moesin

机译：Moesin的主要细胞特异性表达展示的胃壁组织的新颖见解

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摘要

ERM (ezrin, radixin, and moesin) proteins play critical roles in epithelial and endothelial cell polarity, among other functions. In gastric glands, ezrin is mainly expressed in acid-secreting parietal cells, but not in mucous neck cells or zymogenic chief cells. In looking for other ERM proteins, moesin was found lining the lumen of much of the gastric gland, but it was not expressed in parietal cells. No significant radixin expression was detected in the gastric glands. Moesin showed an increased gradient of expression from the neck to the base of the glands. In addition, the staining pattern of moesin revealed a branched morphology for the gastric lumen. This pattern of short branches extending from the glandular lumen was confirmed by using antibody against zonula occludens-1 (ZO-1) to stain tight junctions. With a mucous neck cell probe (lectin GSII, from Griffonia simplicifolia) and a chief cell marker (pepsinogen C), immunohistochemistry revealed that the mucous neck cells at the top of the glands do not express moesin, but, progressing toward the base, mucous cells showing decreased GSII staining had low or moderate level of moesin expression. The level of moesin expression continued to increase toward the base of the glands and reached a plateau in the base where chief cells and parietal cells abound. The level of pepsinogen expression also increased toward the base. Pepsinogen C was located on cytoplasmic granules and/or more generally distributed in chief cells, whereas moesin was exclusively expressed on the apical membrane. This is a clear demonstration of distinctive cellular expression of two ERM family members in the same tissue. The results provide the first evidence that moesin is involved in the cell biology of chief cells. Novel insights on gastric gland morphology revealed by the moesin and ZO-1 staining provide the basis for a model of cell maturation and migration within the gland. Copyright ?2009 the American Physiological Society.
机译：ERM（Ezrin，radixin和Moesin）蛋白质在上皮和内皮细胞极性中起重要作用，以及其他功能。在胃髓中，Ezrin主要在酸分泌的榫眼细胞中表达，但不是在粘液细胞或酶原中的主要细胞中。在寻找其他ERM蛋白时，发现Moesin含有大部分胃腺的内腔，但它在顶叶细胞中没有表达。在胃髓中没有检测到明显的肌瘤表达。 Moesin显示从颈部到腺体的颈部的表达梯度增加。此外，Moesin的染色模式显示出胃腔的支化形态。通过使用针对Zonula occludens-1（ZO-1）的抗体来确认从腺体腔延伸的短分支图案以染色紧密的连接。用粘液细胞探针（来自Griffonia Simplecifolia的凝集素）和主要细胞标记物（胃蛋白酶原C），免疫组化显示，腺体顶部的粘液颈部细胞不表达MoSin，但是朝向碱，粘液进展显示降低的GSII染色的细胞具有低或中等的MOESIN表达。 Moesin表达水平持续朝向腺体的基础增加，并在基础上达到了高原，其中主要细胞和椎管细胞比比皆是。胃蛋白酶原表达水平也朝向基础增加。胃蛋白酶原c位于细胞质颗粒上和/或更普遍分布在主要细胞中，而Moesin仅在顶端膜上表达。这是同一组织中两个ERM家族成员的独特细胞表达的明确证明。结果提供了第一种证据，即Moesin参与了主要细胞的细胞生物学。 MOESIN和ZO-1染色显示的胃腺形态的新洞察力为腺体内细胞成熟和迁移模型提供了基础。版权所有？2009年美国生理社会。

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