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首页> 外文期刊>Journal of the Association for Research in Otolaryngology: JARO >Oncomodulin Expression Reveals New Insights into the Cellular Organization of the Murine Utricle Striola
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Oncomodulin Expression Reveals New Insights into the Cellular Organization of the Murine Utricle Striola

机译：载体载体表达揭示了鼠尿嘧啶striola细胞组织的新见解

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摘要

Oncomodulin (OCM, aka beta-parvalbumin) is an EF-hand calcium binding protein that is expressed in a restricted set of hair cells in the peristriolar region of the mammalian utricle. In the present study, we determined the topologic distribution of OCM among hair cell phenotypes to advance our understanding of the cellular organization of the striola and the relationship of these phenotypes with characteristics of tissue polarity. The distributions of OCM-positive (OCM+) hair cells were quantified in utricles of mature C57Bl/6 mice. Immunohistochemistry was conducted using antibodies to OCM, calretinin, and beta 3-tubulin. Fluorophore-conjugated phalloidin was used to label hair cell stereocilia, which provided the basis for determining hair cell counts and morphologic polarizations. We found OCM expression in striolar types I and II hair cells, though the distributions were dissimilar to the native striolar type I and II distributions, favoring type I hair cells. The distribution of OCM immunoreactivity among striolar type I hair cells also reflected nonrandom distribution among type I-c and I-d phenotypes (i.e., those receiving calretinin-positive and calretinin-negative calyces, respectively). However, many OCM+ hair cells were found lateral to the striola, and within the epithelial region encompassing OCM+ hair cells, the distributions of OCM+ types I-c and I-d hair cells were similar to the native distributions of I-c and I-d in this region. Summarily, these data provide a quantitative perspective supporting the existence of different underlying factors driving the topologic expression of OCM in hair cells than those responsible for tissue polarity characteristics associated within the utricular striola, including calretinin expression in afferent calyces.
机译：on Comodulin（OCM，AKAβ-帕瓦尔蛋白）是EF-HAND钙结合蛋白，其在哺乳动物Utticle的蠕动区域的受限制的一组毛细胞中表达。在本研究中，我们确定了头发细胞表型中OCM的拓扑分布，以推进我们对术细胞组织细胞组织的理解和这些表型与组织极性特征的关系。在成熟C57BL / 6小鼠的尿蛋白中定量了OCM阳性（OCM +）毛细胞的分布。使用抗OCM，Calretinin和Beta 3-微管蛋白进行免疫组织化学。荧光团共轭的阴素用于标记毛细胞立体菌，为确定毛细胞计数和形态学偏振的基础提供了基础。我们在Striarar类型I和II毛细胞中发现了OCM表达，尽管分布与天然Strivar型I和II分布不同，有利于I型毛细胞。 STRIALAR I型毛细胞之间的OCM免疫反应性的分布还反映了I-C型和I-D表型的非random分布（即，分别接受Calretinin-阳性和Calretinin-Dieval Calyces）。然而，许多OCM +毛细胞被发现横向于锶，并且在包围OCM +毛细胞的上皮区域内，OCM +类型I-C和I-D头发细胞的分布类似于该区域I-C和I-D的天然分布。总而言之，这些数据提供了一种定量的透视，其支持不同潜在因素的存在，其推动毛细胞中OCM的拓扑表达而不是负责患有患有的组织极性特性的那些，包括在传入的Calyces中的Caltretinin表达。

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