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首页> 美国卫生研究院文献>JARO: Journal of the Association for Research in Otolaryngology >Oncomodulin Expression Reveals New Insights into the Cellular Organization of the Murine Utricle Striola
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Oncomodulin Expression Reveals New Insights into the Cellular Organization of the Murine Utricle Striola

机译：Oncomodulin表达揭示了对小鼠尿U纹细胞结构的新见解。

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Oncomodulin (OCM, aka β-parvalbumin) is an EF-hand calcium binding protein that is expressed in a restricted set of hair cells in the peristriolar region of the mammalian utricle. In the present study, we determined the topologic distribution of OCM among hair cell phenotypes to advance our understanding of the cellular organization of the striola and the relationship of these phenotypes with characteristics of tissue polarity. The distributions of OCM-positive (OCM+) hair cells were quantified in utricles of mature C57Bl/6 mice. Immunohistochemistry was conducted using antibodies to OCM, calretinin, and β3-tubulin. Fluorophore-conjugated phalloidin was used to label hair cell stereocilia, which provided the basis for determining hair cell counts and morphologic polarizations. We found OCM expression in striolar types I and II hair cells, though the distributions were dissimilar to the native striolar type I and II distributions, favoring type I hair cells. The distribution of OCM immunoreactivity among striolar type I hair cells also reflected nonrandom distribution among type Ic and Id phenotypes (i.e., those receiving calretinin-positive and calretinin-negative calyces, respectively). However, many OCM+ hair cells were found lateral to the striola, and within the epithelial region encompassing OCM+ hair cells, the distributions of OCM+ types Ic and Id hair cells were similar to the native distributions of Ic and Id in this region. Summarily, these data provide a quantitative perspective supporting the existence of different underlying factors driving the topologic expression of OCM in hair cells than those responsible for tissue polarity characteristics associated within the utricular striola, including calretinin expression in afferent calyces.
机译：癌调节蛋白（OCM，又名β-小白蛋白）是一种EF手钙结合蛋白，在哺乳动物尿囊的周毛区域的一组受限毛细胞中表达。在本研究中，我们确定了毛细胞表型之间OCM的拓扑分布，以增进我们对纹状体的细胞组织以及这些表型与组织极性特征之间关系的了解。在成熟的C57Bl / 6小鼠的囊中定量OCM阳性（OCM +）毛细胞的分布。使用针对OCM，钙调蛋白和β3-微管蛋白的抗体进行免疫组织化学。荧光团缀合的鬼笔环肽被用于标记毛细胞立体纤毛，这为确定毛细胞计数和形态极化提供了基础。我们发现ICM和I型毛细血管中的OCM表达，尽管其分布与天然I和II型毛细血管的分布不同，这有利于I型毛发。 OCM免疫反应性在I型毛状毛发细胞中的分布也反映了Ic型和Id型表型（即分别接受钙网蛋白阳性和钙网蛋白阴性calyces的表型）之间的非随机分布。但是，发现许多OCM +毛发细胞位于纹状体外侧，并且在包括OCM +毛发细胞的上皮区域内，OCM + Ic和Id型毛发细胞的分布与该区域中Ic和Id的天然分布相似。综上，这些数据提供了定量的观点，支持了毛细胞中OCM拓扑表达的不同潜在因素的存在，而不是引起房纹内组织相关极性特征的因素的影响，包括传入大肠中钙调蛋白的表达。

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