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首页> 外文期刊>Angewandte Chemie >Mass Spectrometry Signal Amplification Method for Attomolar Detection of Antigens Using SmalL-Molecule-Tagged Gold Microparticles
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Mass Spectrometry Signal Amplification Method for Attomolar Detection of Antigens Using SmalL-Molecule-Tagged Gold Microparticles

机译：用小分子标记的金微粒对抗原抗原检测的质谱信号放大方法

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摘要

Biologically functionalized surfaces are applied widely in biochemical/biological research. Many biochemical and biological events can be detected accurately and reliably on biosurfaces, including a variety of biochips and bio-(nano)materials. Recently, enormous emphasis has been placed on the development of new bioanalytical methods to detect ultralow-abundancy analytes and to handle crude samples, such as blood or urine, without pretreatment. Such demands necessitate the amplification of obtained signals, for example, the polymerase chain reaction (PCR) in the case of oligonucleotide targets and label-free detection formats for observing intact analytes. Nam et al. reported an ultrasensitive protein detection method in which antibodies on microparticles and nanoparticles captured target proteins selectively. The large excess of oligonucleotides on the nanoparticle was applied to the detection of target proteins, and additional PCR provided increased sensitivity to concentrations of several attomoles. Zhao et al. described femtomo-lar detection of DNA utilizing dye-doped silica nanoparticles, which entrapped several fluorophores and therefore enhanced the fluorescent signals resulting from DNA-binding events on the DNA chip.
机译：生物官能化表面广泛应用于生物化学/生物学研究。可以在生物腐植物上准确可靠地检测许多生物化学和生物事件，包括各种生物芯片和生物（纳米）材料。最近，巨大的重点是开发新的生物分析方法，以检测超级丰富分析物，处理粗样品，如血液或尿液，而无需预处理。这种需求需要在寡核苷酸靶标和无标记检测格式的情况下，在寡核苷酸靶标和无标签检测格式的情况下，使得获得的信号的扩增。 Nam等人。报道了超敏蛋白质检测方法，其中微粒和纳米颗粒上的抗体选择性地捕获靶蛋白。将纳米颗粒上的大量过量寡核苷酸施用于靶蛋白的检测，另外的PCR为几个抗体的浓度提供了增加的敏感性。赵等人。描述利用染料掺杂二氧化硅纳米粒子的DNA的毫微微瘤检测，其捕获了几种荧光团，因此增强了由DNA芯片上的DNA结合事件产生的荧光信号。

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