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首页> 外文期刊>Applied Microbiology and Biotechnology >Understanding lipogenesis by dynamically profiling transcriptional activity of lipogenic promoters in Yarrowia lipolytica
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Understanding lipogenesis by dynamically profiling transcriptional activity of lipogenic promoters in Yarrowia lipolytica

机译：通过动态地分析乳头吡酰吡啶的脂质促进剂的转录活性来了解脂肪生成

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摘要

Lipogenesis is a complicated process involving global transcriptional reprogramming of lipogenic pathways. It is commonly believed that nitrogen starvation triggers a metabolic shift that reroutes carbon flux from Krebs cycles to lipogenesis. In this study, we systematically surveyed and dynamically profiled the transcriptional activity of 22 lipogenic promoters aiming to delineate a picture how nitrogen starvation regulates lipogenesis in Y. lipolytica. These lipogenic promoters drive the expression of critical pathways that are responsible for the generation of reducing equivalents (NADPH), carbon backbones (acetyl-CoA, malonyl-CoA, DHAP, etc.), synthesis and degradation of fatty acids. Specifically, our investigated promoters span across an array of metabolic pathways, including glycolysis, Krebs cycle, pentose phosphate pathway, mannitol cycle, glutamine-GABA cycle, fatty acid and lipid synthesis, glyoxylate, -oxidation, and POM (pyruvate-oxaloacetate-malate) cycle. Our work provides evidences that mannitol cycle, glutamine-GABA cycle and amino acid degradation, pyruvate oxidation, and acetate assimilation pathways are lipogenesis-related steps involved in generating cytosolic NADPH and acetyl-CoA precursors. This systematic investigation and dynamic profiling of lipogenic promoters may help us better understand lipogenesis, facilitate the formulation of structure-based kinetic models, as well as develop efficient cell factories for fuels and chemical production in oleaginous species.
机译：脂肪生成是一种复杂的过程，涉及全局转录重编程的脂肪原途径。通常认为氮饥饿触发了将碳通量从克雷斯循环转化为脂肪生成的代谢移位。在这项研究中，我们系统地进行了调查和动态地分析了22例富脂质启动子的转录活性，旨在描绘氮饥饿如何调节Y. Lipolytica中的脂肪生成。这些脂肪原促促进剂驱动临界途径的表达，该临界途径负责产生还原当量（NADPH），碳骨架（乙酰-COA，丙酰基-COA，DHAP等），合成和降解脂肪酸。具体而言，我们的调查启动子跨越一系列代谢途径，包括糖醇分解，克雷布循环，戊糖途径，甘露糖醇循环，谷氨酰胺-GABA循环，脂肪酸和脂质合成，乙醛酸，---氧化和POM（丙酮酸 - 草乙酸盐 - 苹果酸酯） ） 循环。我们的作品提供了甘露醇循环，谷氨酰胺-GABA循环和氨基酸降解，丙酮酸氧化和醋酸酸的同化途径是产生细胞溶质NADPH和乙酰-COA前体的脂肪生成相关步骤。这种系统的调查和动态剖析的脂质促进剂可以帮助我们更好地了解脂肪生成，便于制定基于结构的动力学模型，以及在含油物种中开发有效的细胞厂进行燃料和化学生产。

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