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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Effects of the Subaleurone Layer of Rice on Macrophage Activation and Protection of Pollen Allergy in a Murine Model
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Effects of the Subaleurone Layer of Rice on Macrophage Activation and Protection of Pollen Allergy in a Murine Model

机译：水稻硫代酮层对鼠模型巨噬细胞激活和花粉过敏的影响

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Background/Aim: Oral intake of lipopolysaccharide (LPS) has been demonstrated to be effective in the prevention of various diseases. We have found that the subaleurone layer of rice contains a large amount of LPS. The aim of this study was to evaluate the role of this layer in innate immunity. Materials and Methods: Using the Saika-style rice polishing process, a sbaleurone layer and the rice retaining a subaleurone layer and polished white rice were prepared from brown rice. Using hot-water extracts from rice, LPS content was measured by the Limulus reaction and the effect of activation of macrophages was evaluated on the basis of their phagocytic activity and nitric monoxide (NO) and tumor necrosis factor (TNF) production levels. Toll-like receptor (TLR)-2-, TLR-4- and TLR-9-transfected human embryonic kidney (HEK) cells were used to identify the activation pathway. An allergy mouse model was used to evaluate the prevention of pollen allergy. Results: When compared to polished white rice, rice retaining a subaleurone layer had a 6-fold increase in LPS and an increased macrophage activation when phagocytic activity and NO and TNF production were used as indices. TRL4 was the major pathway for such activation. Anti-allergy test by oral intake of subaleurone showed a significant preventive effect for pollen allergy. Conclusion: Compared to polished white rice, rice retaining a subaleurone layer contained a high level of LPS with higher macrophage activation. Furthermore, oral administration of the rice demonstrated a preventive effect for pollen allergy, thus indicating its utility as a functional food that has a regulatory effect on innate immunity.
机译：背景/目的：脂多糖（LPS）的口服摄入量已被证明是有效预防各种疾病。我们发现，水稻的亚麦酮层含有大量的LPS。本研究的目的是评估该层在先天免疫中的作用。材料和方法：采用氧木粉型水稻抛光工艺，从糙米制备SBaleurone层和水稻保留子霉酮层和抛光白米。利用水稻的热水提取物，通过硫化反应测量LPS含量，并根据其吞噬活性和一硝基一氧化氮（NO）和肿瘤坏死因子（TNF）产生水平评估巨噬细胞活化的效果。使用Toll样受体（TLR）-2-，TLR-4-和TLR-9转染的人胚胎肾（HEK）细胞用于鉴定活化途径。过敏的小鼠模型用于评估预防花粉过敏。结果：与抛光白米相比，当吞噬活性和NO和TNF生产用作指数时，LPS的LPS增加6倍，巨噬细胞活化增加，增加了巨噬细胞活化。 TRL4是这种激活的主要途径。口服摄入子石的抗过敏试验对花粉过敏进行了显着的预防效果。结论：与抛光白米相比，水稻保持子霉酮层含有高水平的LPS，具有较高的巨噬细胞活化。此外，水稻口服给药对花粉过敏的预防效果，从而表明其作为对先天免疫具有监管影响的功能性的效用。

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