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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >The Effect of Cold Atmospheric Plasma on the Membrane Permeability of Human Osteosarcoma Cells
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The Effect of Cold Atmospheric Plasma on the Membrane Permeability of Human Osteosarcoma Cells

机译：冷大气等离子体对人骨骨膜细胞膜渗透性的影响

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摘要

Background: Cold atmospheric plasma (CAP) has a variety of anticancer effects on different cancer cell types. In osteosarcoma (OS) cells, CAP reduces growth and motility, induces apoptosis, and alters secretion of cellular factors. The influence of CAP on membrane integrity of OS cells is unknown. Materials and Methods: Two different OS cell lines (U-2 OS and MNNG-HOS) were treated with CAP. Proliferation assays for cell growth after treatment was performed. Alterations in membrane permeability and the associated translocation of low molecular weight particles through the cytoplasmic membrane of OS cells after CAP treatment were shown in fluorescein diacetate (FDA) assays. Results: FDA increasingly passed the membrane after CAP treatment and this effect depended on the duration of treatment. It was also shown that after CAP treatment, FDA was able to diffuse into the cells from the outside as well as out of the cell interior. These effects were observed when CAP-treated buffer was used and therefore no direct contact between cells and CAP occurred. Conclusion: The observations suggest that changes in membrane permeability and function may contribute to the antiproliferative effects of CAP.
机译：背景：冷大气血浆（帽）对不同癌细胞类型具有各种抗癌作用。在骨肉瘤（OS）细胞中，帽减少了生长和运动，诱导细胞凋亡，并改变细胞因子的分泌。帽对OS细胞膜完整性的影响是未知的。材料和方法：用帽处理两种不同的OS细胞系（U-2 OS和MNNG-HOS）。进行治疗后细胞生长的增殖测定。在荧光素二乙酸酯（FDA）测定中，显示膜渗透性通过OS细胞的细胞质膜的膜渗透性和低分子量颗粒的相关易位。结果：FDA在盖子处理后越来越多地通过膜，并且这种效果取决于治疗持续时间。还表明，在盖帽治疗后，FDA能够从外部散布到细胞中，也能够从细胞内部扩散到细胞中。当使用帽处理的缓冲液时，观察到这些效果，因此不会发生细胞和帽之间的直接接触。结论：观察结果表明膜渗透性和功能的变化可能有助于帽的抗增殖作用。

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