• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology Progress >Characterization of three-dimensional rat central nervous system culture maturation, with applications to monitor cholinergic integrity
【24h】

Characterization of three-dimensional rat central nervous system culture maturation, with applications to monitor cholinergic integrity

机译：三维大鼠中枢神经系统培养成熟的特征，具有监测胆碱能完整性的应用

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Studying age-related neuropathologies in vitro requires a three-dimensional (3D) culture system presenting mature phenotypes. In this study, we aimed to determine whether aged reaggregate cultures physiologically represent mature brain tissue. Results support that embryo-derived rat central nervous system (CNS) reaggregate cultures develop into mature-like tissues, comparable to in vivo maturation, including the following characteristics: (a) progressive reduction in cell proliferation (reduced anti-Ki-67 immunoreactivity), (b) progressive restriction of long neurite growth potential (as explant cultures), and (c) increased and sustained synaptic enzyme (acetylcholine esterase, AChE) activity. The acquisition of mature-like reaggregate cultures has allowed us to pursue the hypothesis that the physiological integrity of 3D CNS cultures may be monitored by synaptic enzyme activity. To assess this hypothesis, mature-like reaggregates were exposed to H2O2, glutamate, or amyloid beta(1-42); each resulted in diminished AChE activity. H2O2 exposure resulted in nuclear fragmentation. Glutamate and amyloid beta(1-42) exposure resulted in acetylcholine content reduction. Simultaneous reduction of AChE activity and acetylcholine content verified diminished cholinergic integrity. This scheme exploiting synapse enzyme activity of mature-like 3D CNS tissue is therefore applicable to age-related neuropathology research including in vitro screening of conditions potentially affecting synapse integrity, including the promotion of dementia.
机译：在体外研究与年龄相关的神经病变需要呈现成熟表型的三维（3D）培养系统。在这项研究中，我们旨在确定老化的重新生成培养物生理学上是否代表成熟的脑组织。结果支持胚胎衍生的大鼠中枢神经系统（CNS）将培养物发生在成熟的组织中，与体内成熟相当，包括以下特征：（a）细胞增殖的逐步降低（降低抗Ki-67免疫反应性） （b）长期神经态生长潜力（作为外植体培养）的逐步限制，（c）增加和持续突触酶（乙酰胆碱酯酶，疼痛）活性。获取类似成熟的重新生成培养物使我们能够追求假设，即可以通过突触酶活性监测3D CNS培养物的生理完整性。为了评估该假设，将成熟的重新凝集暴露于H 2 O 2，谷氨酸或淀粉样蛋白β（1-42）;每个都导致疼痛活动减少。 H2O2暴露导致核碎裂。谷氨酸和淀粉样蛋白β（1-42）暴露导致乙酰胆碱含量降低。同时降低疼痛活性和乙酰胆碱含量验证了胆碱能完整性的减少。因此，该方案利用成熟3D CNS组织的突触酶活性，适用于与年龄相关的神经病理学研究，包括体外筛查可能影响突触完整性的病症，包括促进痴呆症。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号