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首页> 外文期刊>Biotechnology Progress >The use of catechins in Chinese hamster ovary cell media for the improvement of monoclonal antibody yields and a reduction of acidic species
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The use of catechins in Chinese hamster ovary cell media for the improvement of monoclonal antibody yields and a reduction of acidic species

机译：在中国仓鼠卵巢细胞培养基中使用儿茶素，改善单克隆抗体产量和酸性物种的减少

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摘要

Catechin compounds have potential benefits for recombinant monoclonal antibody (Mab) production as chemical additives in cell culture media. In this study, four catechin compounds catechin (Cat), epicatechin (EC), gallocatechin-gallate (GCG), and epigallocatechin-gallate (EGCG) were added to cell culture media (at 50 mu M) and their effects on the recombinant Chinese hamster ovary (CHO) cell culture, specific productivity, and Mab quality were assessed. The results indicate that the improvement of specific productivity was linked to cell growth inhibition. All catechins caused cell phase growth arrest by lowering the number of cells in the G1/G0 phase and increasing the cells in the S and G2/M phases. Late addition of the catechin resulted in a significantly higher final IgG concentration. Cat and EC caused an improvement in the final antibody titer of 1.5 +/- 0.1 and 1.3 +/- 0.1 fold, respectively. Catechins with a galloyl group (GCG and EGCG) arrested cell growth and reduced cell specific productivity at the concentrations tested. The Cat-treated IgG was found to have reduced acidic species with a corresponding increase in the main peak.
机译：儿茶素化合物具有重组单克隆抗体（MAB）生产作为细胞培养基中的化学添加剂的潜在益处。在该研究中，将四种儿茶素化合物儿茶素（猫），EPICATECHIN（EC），GallocateChin-Gallate（GCG）和EpigallocateChin-Gallate（EGCG）加入细胞培养基（50μm）中，它们对重组中文的影响评估仓鼠卵巢（CHO）细胞培养，具体生产力和MAB质量。结果表明，提高比生产率与细胞生长抑制有关。所有儿茶素通过降低G1 / G0相中的细胞数并增加S和G2 / m相中的细胞来引起细胞相生长滞留。儿茶素的晚期添加导致最终的IgG浓度明显更高。 CAT和EC分别导致最终抗体滴度为1.5 +/- 0.1和1.3 +/- 0.1折的改善。在测试的浓度上被阻止细胞生长和细胞比生产率降低的儿茶素。发现猫处理的IgG具有降低的酸性物质，主要峰值相应增加。

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