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首页> 外文期刊>Analytical Biochemistry: An International Journal of Analytical and Preparative Methods >Comparative determination of some phytohormones in wild-type and genetically modified plants by gas chromatography-mass spectrometry and high-performance liquid chromatography-tandem mass spectrometry
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Comparative determination of some phytohormones in wild-type and genetically modified plants by gas chromatography-mass spectrometry and high-performance liquid chromatography-tandem mass spectrometry

机译：气相色谱-质谱和高效液相色谱-串联质谱法比较测定野生型和转基因植物中的某些植物激素

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摘要

The analytical performances of two optimized analytical methodologies used for the determination of auxins, cytokinins, and abscisic acid in plant samples were critically compared. Phytohormones were extracted from Nicotiana glauca samples using a modified Bieleski solvent and determined both by gas chromatography-mass spectrometry (GC-MS), after derivatization with N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA), and by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) on the Bieleski extract without any further treatment. HPLC-MS/MS gave better results in terms of higher coefficients of determination of the calibration curves, higher and more reproducible recoveries, lower limits of detection, faster sample preparation, and higher sample throughput. Thus, two sets of N. glauca and N. langsdorffii samples, both wild-type and genetically modified by inserting the glucocorticoid receptor (GR) gene encoding for the rat glucocorticoid receptor, were first characterized by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis and then analyzed by HPLC-MS/MS. Significant differences in the phytohormone content between the two sample sets were found and are very important in terms of understanding the mechanisms and effects on growth processes and the development of transgenic plants.
机译：严格比较了用于测定植物样品中植物生长素，细胞分裂素和脱落酸的两种优化分析方法的分析性能。 N，O-双（三甲基甲硅烷基）三氟乙酰胺（BSTFA）衍生化后，使用改良的Bieleski溶剂从青烟草样品中提取植物激素，并通过气相色谱-质谱（GC-MS）测定-Bieleski提取物进行串联质谱分析（HPLC-MS / MS），无需任何进一步处理。 HPLC-MS / MS在更高的校准曲线测定系数，更高和更高重现性的回收率，更低的检测限，更快的样品制备和更高的样品通量方面提供了更好的结果。因此，首先通过逆转录酶-聚合酶链反应（RT- PCR）分析，然后通过HPLC-MS / MS分析。发现两个样品组之间植物激素含量的显着差异，这对于理解生长过程的机制和影响以及转基因植物的发育非常重要。

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