Effect of UV irradiation on aflatoxin reduction: a cytotoxicity evaluation study using human hepatoma cell line

Patras Ankit; Julakanti Sharath; Yannam Sudheer; Bansode Rishipal R.; Burns Mallory; Vergne Matthew J.

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Effect of UV irradiation on aflatoxin reduction: a cytotoxicity evaluation study using human hepatoma cell line

机译：紫外线辐照对黄曲霉毒素减少的影响：使用人肝细胞系的细胞毒性评价研究

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In this proof-of-concept study, the efficacy of a medium-pressure UV (MPUV) lamp source to reduce the concentrations of aflatoxin B-1, aflatoxin B-2, and aflatoxin G(1) (AFB(1,) AFB(2), and AFG(1)) in pure water is investigated. Irradiation experiments were conducted using a collimated beam system operating between 200 to 360 nm. The optical absorbance of the solution and the irradiance of the lamp are considered in calculating the average fluence rate. Based on these factors, the UV dose was quantified as a product of average fluence rate and treatment time. Known concentrations of aflatoxins were spiked in water and irradiated at UV doses ranging from 0, 1.22, 2.44, 3.66, and 4.88 J cm(-2). The concentration of aflatoxins was determined by HPLC with fluorescence detection. LC-MS/MS product ion scans were used to identify and semi-quantify degraded products of AFB(1), AFB(2), and AFG(1). It was observed that UV irradiation significantly reduced aflatoxins in pure water (p 0.05). Irradiation doses of 4.88 J cm(-2) reduced concentrations 67.22% for AFG(1), 29.77% for AFB(2), and 98.25% for AFB(1) (p 0.05). Using this technique, an overall reduction of total aflatoxin content of approximate to 95% (p 0.05) was achieved. We hypothesize that the formation of OH radicals initiated by UV light may have caused photolysis of AFB(1), AFB(2), and AFG(1) molecules. In cell culture studies, our results demonstrated that the increase of UV dosage decreased the aflatoxin-induced cytotoxicity in HepG(2) cells. Therefore, our research finding suggests that UV irradiation can be used as an effective technique for the reduction of aflatoxins.

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来源
《Mycotoxin Research》 |2017年第4期|共8页
作者
Patras Ankit; Julakanti Sharath; Yannam Sudheer; Bansode Rishipal R.; Burns Mallory; Vergne Matthew J.;
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作者单位

Tennessee State Univ Dept Agr &

Environm Sci Food Biosci &

Technol Program Nashville TN 37209 USA;

Tennessee State Univ Dept Agr &

Environm Sci Food Biosci &

Technol Program Nashville TN 37209 USA;

Tennessee State Univ Dept Agr &

Environm Sci Food Biosci &

Technol Program Nashville TN 37209 USA;

North Carolina A&

T State Univ Ctr Excellence Postharvest Technol North Carolina Res Campus Kannapolis NC USA;

Lipscomb Univ Dept Pharmaceut Sci Nashville TN 37204 USA;

Lipscomb Univ Dept Pharmaceut Sci Nashville TN 37204 USA;

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原文格式 PDF
正文语种 eng
中图分类 Q939.5;
关键词
Aflatoxins; UVirradiation; Toxicity; Dose;

机译：黄曲霉毒素;UVIARRADIATION;毒性;剂量;

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6. Effect of UV irradiation on aflatoxin reduction: a cytotoxicity evaluation study using human hepatoma cell line [J] . Patras Ankit, Julakanti Sharath, Yannam Sudheer, Mycotoxin Research . 2017,第4期

机译：紫外线辐照对黄曲霉毒素减少的影响：使用人肝细胞系的细胞毒性评价研究
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Effect of UV irradiation on aflatoxin reduction: a cytotoxicity evaluation study using human hepatoma cell line

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