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首页> 外文期刊>Journal of applied toxicology >Exposure to 4-nonylphenol induces a shift in the gene expression ofgsdfand testis-ova formation and sex reversal in Japanese medaka (Oryzias latipes)
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Exposure to 4-nonylphenol induces a shift in the gene expression ofgsdfand testis-ova formation and sex reversal in Japanese medaka (Oryzias latipes)

机译：暴露于4-壬基酚，诱导日本Medaka（奥西亚山脉）的Gegsdfand Testis-OVA形成和性逆转的转变

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The branched isomer mixture 4-nonylphenol (4-NP) has been used worldwide as a surfactant, and can have endocrine-disrupting effects on aquatic organisms. For instance, 4-NP induces the formation of testis-ova (i.e., testicular and ovarian tissue in the same gonad) or male to female sex reversal of various teleost fishes. Recently, our group revealed that alteredgsdfgene expression is associated with disruption of gonadal differentiation in Japanese medaka (Oryzias latipes) embryos exposed to methyltestosterone or bisphenol A, suggesting thatgsdfmight be useful as a biomarker for predicting the impact of endocrine-disrupting chemicals (EDCs) on gonadal differentiation. Here, we used 4-NP to examine further whethergsdfexpression at the embryo stage is useful for predicting EDC impact on gonadal sex differentiation. When fertilized medaka eggs were exposed to 32 or 100 mu g/L 4-NP, testis-ova in genetic males and sex reversal from genetic male to phenotypic female were observed. At stage 38 (just before hatching), 4-NP exposure at 1-100 mu g/L did not affectgsdfexpression in XX embryos compared with the nontreated control; however, in XY embryos, thegsdfexpression in the 100 mu g/L-exposed group was significantly lower than that in the controls. The 4-NP concentration at whichgsdfexpression was suppressed was equal to that at which testis-ova and sex reversal were induced. These results indicate that expression of thegsdfgene at the embryonic stage in medaka is a useful biomarker for predicting the impact of EDCs on sexual differentiation.
机译：支链异构体混合物4-壬基酚（4-NP）已在世界范围内用作表面活性剂，可对水生生物产生内分泌干扰作用。例如，4-NP诱导各种硬骨鱼类形成睾丸卵子（即同一性腺中的睾丸和卵巢组织）或雄性到雌性的性反转。最近，我们的研究小组发现，在暴露于甲基睾酮或双酚A的日本medaka（Oryzias latipes）胚胎中，SDFG基因表达的改变与性腺分化的破坏有关，这表明GSDF可能是预测内分泌干扰物（EDC）对性腺分化影响的有用生物标记物。在这里，我们使用4-NP进一步检测胚胎期GSDF表达是否有助于预测EDC对性腺性别分化的影响。当受精的medaka卵暴露于32或100μg/L 4-NP时，观察到遗传雄性的睾丸卵子以及从遗传雄性到表型雌性的性反转。在38期（孵化前），与未处理对照组相比，1-100μg/L的4-NP暴露对XX胚胎中GSDF的表达没有影响；然而，在XY胚胎中，100μg/L暴露组的GSDF表达显著低于对照组。抑制GSDF表达的4-NP浓度与诱导睾丸ova和性反转的浓度相同。这些结果表明，在medaka胚胎阶段GSDF基因的表达是预测EDC对性分化影响的有用生物标志物。

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