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首页> 外文期刊>Annals of Agricultural Science >Production of transgenic banana plants conferring tolerance to salt stress.
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Production of transgenic banana plants conferring tolerance to salt stress.

机译：赋予耐盐胁迫能力的转基因香蕉植物的生产。

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Production of bananas is limited in areas that have soils with excess sodium. In this study, a transformation system in banana Grand Nain cultivar was established using the apical meristem explant and plasmid pAB6 containing the herbicide-resistant gene (bar) as a selectable marker and gus reporter gene. The microprojectile bombardment transformation system using 650 psi was successfully used for introducing the studied genes in banana explants. The expression of the introduced genes was detected using leaf painting and GUS histochemical tests, respectively. The present results showed that among the selection stage, 36.5% of the bombarded explants survived on the BI3 medium supplemented with 3 mg/litre bialaphos, while, 26.6% of the tested explants showed a positive reaction in the GUS assay. To detect the presence of bar and gus genes the PCR was successfully used. These results encourage the idea of possibility of banana crop improvement using in vitro technique through microprojectile bombardment. Therefore, the plasmid pNM1 that carries the bar and P5CS ( Delta 1-pyrroline-5-carboxylate synthetase for proline accumulation) genes was introduced in banana Grand Nain cultivar to produce transgenic plants expressing the salt tolerance gene. Results showed that the majority of herbicide-resistant banana plantlets were successfully acclimatized. In studying the effects of different salt concentrations on the produced transgenic banana plants, results showed lower decrease in the percentage of survived plants, pseudostem diameter and leaf area with an increase of salt concentrations in case of transgenic plants compared with the controls..
机译：在土壤中钠含量过多的地区，香蕉的产量受到限制。在这项研究中，使用顶端分生组织外植体和含有抗除草剂基因（bar）作为选择标记和gus报道基因的质粒pAB6，建立了香蕉大Nain品种的转化系统。使用650 psi的微粒轰击转化系统已成功用于将研究的基因引入香蕉外植体中。分别使用叶画法和GUS组织化学测试检测导入基因的表达。目前的结果表明，在选择阶段中，有36.5％的轰击外植体在添加了3 mg / L双丙氨磷的BI3培养基上存活，而26.6％的被测试外植体在GUS分析中显示出阳性反应。为了检测bar和gus基因的存在，成功地使用了PCR。这些结果鼓舞了通过微粒轰击使用体外技术改良香蕉作物的想法。因此，将携带bar和P5CS（用于脯氨酸积累的δ1-吡咯啉-5-羧酸合成酶）基因的质粒pNM1引入香蕉Grand Nain品种中，以产生表达耐盐基因的转基因植物。结果表明，大多数抗除草剂香蕉苗均已成功适应。在研究不同盐浓度对生产的转基因香蕉植物的影响时，与对照相比，结果表明，在盐浓度增加的情况下，随着盐浓度的增加，存活植物的百分比，假茎直径和叶面积的降低降低。

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