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首页> 外文期刊>Antonie van Leeuwenhoek: Journal of Microbiology and serology >Comparing activated sludge fungal community population diversity using denaturing gradient gel electrophoresis and terminal restriction fragment length polymorphism
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Comparing activated sludge fungal community population diversity using denaturing gradient gel electrophoresis and terminal restriction fragment length polymorphism

机译：使用变性梯度凝胶电泳和末端限制性片段长度多态性比较活性污泥真菌群落的多样性

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摘要

We compared the relative values of denaturing gradient gel electrophoresis (DGGE) and terminal restriction fragment length polymorphism (T-RFLP) for profiling fungal communities in wastewater treatment plants using both ITS and 18S rRNA gene fragments as phylogenetic markers. A similar number of fungal ribotypes was obtained with both methods for the same treatment plant when the ITS primer set was used, while a greater number of ribotypes was obtained with T-RFLP compared to DGGE with the 18S rRNA primer set. Non-metric multi-dimensional scaling of presence/absence data and analysis of similarity showed that both methods could distinguish between the different plant communities at a statistically significant level (p < 0.05), regardless of which phylogenetic marker was used. The data suggest that both methods can be used preferably together to profile activated sludge fungal communities. A comparison of profiles generated with both these phylogenetic markers based on the number of ribotypes/bands, suggests that the 18S rRNA region is more discriminatory than the ITS region. Detected differences in fungal community compositions between plants probably reflect differences in their influent compositions and operational parameters.
机译：我们比较了变性梯度凝胶电泳（DGGE）和末端限制性片段长度多态性（T-RFLP）在污水处理厂中使用ITS和18S rRNA基因片段作为系统发生标记的真菌群落图谱的相对值。当使用ITS引物组时，对于同一处理厂，两种方法均获得了相似数量的真菌核糖型，而与18S rRNA引物组的DGGE相比，用T-RFLP获得了更多的核糖型。存在/不存在数据的非度量多维标度和相似性分析表明，无论使用哪种系统发生标记，两种方法均可以统计学上显着的水平（p <0.05）区分不同的植物群落。数据表明，两种方法可优选一起用于分析活性污泥真菌群落。根据核糖型/条带数量对这两种系统发育标记生成的图谱进行比较，表明18S rRNA区比ITS区更具区分性。在植物之间检测到的真菌群落组成的差异可能反映了进水成分和操作参数的差异。

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