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首页> 外文期刊>Applied Microbiology and Biotechnology >The redox-sensing protein Rex, a transcriptional regulator of solventogenesis in Clostridium acetobutylicum
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The redox-sensing protein Rex, a transcriptional regulator of solventogenesis in Clostridium acetobutylicum

机译：氧化还原敏感蛋白Rex，丙酮丁醇梭菌中溶剂生成的转录调节剂

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摘要

Solventogenic clostridia are characterised by their biphasic fermentative metabolism, and the main final product n-butanol is of particular industrial interest because it can be used as a superior biofuel. During exponential growth, Clostridium acetobutylicum synthesises acetic and butyric acids which are accompanied by the formation of molecular hydrogen and carbon dioxide. During the stationary phase, the solvents acetone, butanol and ethanol are produced. However, the molecular mechanisms of this metabolic switch are largely unknown so far. In this study, in silico, in vitro and in vivo analyses were performed to elucidate the function of the CAC2713-encoded redox-sensing transcriptional repressor Rex and its role in the solventogenic shift of C. acetobutylicum ATCC 824. Elec-trophoretic mobility shift assays showed that Rex controls the expression of butanol biosynthetic genes as a response to the cellular NADH/NAD+ ratio. Interestingly, the Rex-negative mutant C. acetobutylicum rex:int(95) produced high amounts of ethanol and butanol, while hydrogen and acetone production were significantly reduced. Both ethanol and butanol (but not acetone) formation started clearly earlier than in the wild type. In addition, the rex mutant showed a de-repression of the bifunctional aldehyde/alcohol dehy-drogenase 2 encoded by the adhE2 gene (CAP0035) as demonstrated by increased adhE2 expression as well as high NADH-dependent alcohol dehydrogenase activities. The results presented here clearly indicated that Rex is involved in the redox-dependent solventogenic shift of C. acetobutylicum.
机译：产溶剂的梭状芽胞杆菌的特征在于其双相发酵代谢，主要的最终产物正丁醇具有特殊的工业价值，因为它可以用作优质的生物燃料。在指数增长过程中，丙酮丁醇梭菌合成乙酸和丁酸，并伴随着分子氢和二氧化碳的形成。在固定阶段，生成了丙酮，丁醇和乙醇溶剂。但是，到目前为止，这种代谢转换的分子机制尚不清楚。在这项研究中，进行了计算机，体外和体内分析，以阐明CAC2713编码的氧化还原敏感转录阻遏物Rex的功能及其在丙酮丁醇梭菌ATCC 824溶剂致溶剂化转变中的作用。表明Rex控制了丁醇生物合成基因的表达，作为对细胞NADH / NAD +比例的响应。有趣的是，Rex阴性突变体丙酮丁醇梭菌rex：int（95）产生了大量的乙醇和丁醇，而氢气和丙酮的产生显着减少。乙醇和丁醇（但不是丙酮）的形成都明显早于野生型。此外，rex突变体显示了adhE2基因（CAP0035）编码的双功能醛/醇脱氢酶2的抑制，这可以通过增加adhE2表达以及高NADH依赖性醇脱氢酶活性来证明。这里呈现的结果清楚地表明，Rex参与了丙酮丁醇梭菌的依赖于氧化还原的溶剂转化。

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