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首页> 外文期刊>Applied Microbiology and Biotechnology >Monascus-fermented red mold rice exhibits cytotoxic effect and induces apoptosis on human breast cancer cells
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Monascus-fermented red mold rice exhibits cytotoxic effect and induces apoptosis on human breast cancer cells

机译：红曲霉发酵的红霉米表现出细胞毒性作用并诱导人乳腺癌细胞凋亡

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摘要

Red mold rice (RMR) is a traditional food and folk medicine to Asian people and has recently become a popular health supplement. RMR has been shown to have some anticancer activities, although the mechanism for inducing cell death of human breast cancer cells is still not fully understood. In this study, bioactive extracts of RMR fermented by Monascus purpureus NTU 803 were analyzed for effects on apoptosis induction in human breast cancer cells. The RMR ethanol extract and ethyl acetate extract contain monacolin K, total phenols, and flavonoids, the three components that have been reported to have anticancer activity. Red mold rice extracts (RMRE) exhibited selective cytotoxic effect on MCF-7 cells. RMRE treatment induced apoptosis and cell cycle arrest at G2/M phase. Apoptosis was confirmed by annexin V-fluorescein isothiocyanate (FITC)/propidium iodide staining, the observation of characteristic chromatin condensation, nuclear DNA fragmentation, and poly(ADP-ribose) polymerase cleavage. Furthermore, the RMRE-induced apoptosis in MCF-7 cells may occur through a mitochondria-dependent pathway while triggering an appropriate balance of bax/bcl-2 and activation of caspase-9 and caspase-3 in a time-dependent manner. To conclude, RMRE exhibits direct cytotoxic and proapoptotic effects on MCF-7 cells and could be considered as a potential functional food for breast cancer prevention.
机译：红霉米（RMR）是亚洲人的传统食品和民间药物，最近已成为流行的保健补品。尽管仍未完全了解诱导人类乳腺癌细胞死亡的机制，但RMR具有一定的抗癌活性。在这项研究中，分析了红曲霉NTU 803发酵的RMR的生物活性提取物对人乳腺癌细胞凋亡诱导的影响。 RMR乙醇提取物和乙酸乙酯提取物包含莫纳可林K，总酚和类黄酮，据报道这三种成分具有抗癌活性。红霉米提取物（RMRE）对MCF-7细胞具有选择性的细胞毒性作用。 RMRE处理可诱导G2 / M期细胞凋亡和细胞周期停滞。膜联蛋白V-异硫氰酸荧光素（FITC）/碘化丙啶染色，特征性染色质浓缩，核DNA片段化和聚（ADP-核糖）聚合酶裂解证实了细胞凋亡。此外，RMRE诱导的MCF-7细胞凋亡可能通过线粒体依赖性途径发生，同时以时间依赖性方式触发bax / bcl-2的适当平衡以及caspase-9和caspase-3的激活。总之，RMRE对MCF-7细胞具有直接的细胞毒性和促凋亡作用，可以被认为是预防乳腺癌的潜在功能性食品。

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