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Growth of Photorhabdus luminescens in batch and glucose fed-batch culture

机译:分批和葡萄糖补料分批培养的光生光杆菌

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Photorhabdus luminescens, a bacterial symbiont of entomopathogenic biocontrol nematodes, was grown in batch and glucose fed-batch culture. The cell density, bioluminescence, production of antibiotic substances, number of cells with inclusion bodies, glucose concentration and oxygen uptake rate were recorded. The addition of 12.4 g l(-1) glucose prolonged the growth, and the yield almost doubled, from 6.85 g l(-1) to 12.45 g l(-1) dry mass. The production of antibiotic substances increased by 140%. Bioluminescence was higher in the batch culture. A shift of P. luminescens to phase II variants was not detected. [References: 25]
机译:分批和葡萄糖分批培养培养了光致病菌(Photorhabdus luminescens),它是昆虫病原性生物防治线虫的一种细菌共生体。记录细胞密度,生物发光,抗生素物质的产生,具有包涵体的细胞数,葡萄糖浓度和氧吸收率。添加12.4 g l(-1)葡萄糖可延长生长,产量几乎翻倍,从6.85 g l(-1)增至12.45 g l(-1)干重。抗生素物质的产量增加了140%。分批培养中生物发光较高。未检测到发光夜蛾向II期变体的转变。 [参考:25]

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