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首页> 外文期刊>Applied Microbiology and Biotechnology >Cloning, nucleotide sequence and expression of a hydantoinase and carbamoylase gene from Arthrobacter aurescens DSM 3745 in Escherichia coli and comparison with the corresponding genes from Arthrobacter aurescens DSM 3747
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Cloning, nucleotide sequence and expression of a hydantoinase and carbamoylase gene from Arthrobacter aurescens DSM 3745 in Escherichia coli and comparison with the corresponding genes from Arthrobacter aurescens DSM 3747

机译：金色节杆菌DSM 3745中的乙内酰胺酶和氨基甲酰酶基因的克隆，核苷酸序列和表达，并与金色节杆菌DSM 3747中的相应基因进行比较

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摘要

The genes encoding hydantoinases (hyuH1) and carbamoylases (hyuC1) from Arthrobacter aurescens DSM 3745 and Arthrobacter aurescens DSM 3747 (hyuH2, hyuC2) were cloned in Escherichia coli and the nucleotide sequences determined. The hydantoinase genes comprised 1,377 base pairs and the carbamoylase genes 1,239 base pairs each. Both hydantoinases, as well as both carbamoylases, showed a high degree of nucleotide and amino acid sequence identity (96-98%). The hyuH and hyuC genes were expressed in E. Loll under the control of the rhamnose promoter and the different specific activities obtained in E. coli crude extracts were compared to those produced by the original hosts. For purification the hyuH2 gene was expressed as a maltose-binding protein (MalE) and as an intein-chitin binding domain (CBD) fusion in E. coli. The expression of malE-hyuH2 resulted in the production of more soluble and active protein. With respect to temperature stability, optimal pH and optimal temperature, substrate and ste reospecificity, the purified fusion enzyme exhibited properties similar to those of the wild-type enzyme. [References: 23]
机译：将来自金色节杆菌DSM 3745和金色节杆菌DSM 3747（hyuH2，hyuC2）的编码乙内酰脲酶（hyuH1）和氨基甲酰酶（hyuC1）的基因克隆到大肠杆菌中，并确定核苷酸序列。乙内酰脲酶基因包含1,377个碱基对，而氨甲酰酶基因分别包含1,239个碱基对。两种乙内酰脲酶以及两种氨甲酰酶均显示出高度的核苷酸和氨基酸序列同一性（96-98％）。 hyuH和hyuC基因在鼠李糖启动子的控制下在大肠杆菌中表达，并将在大肠杆菌粗提物中获得的不同比活与原始宿主产生的比活进行了比较。为了纯化，在大肠杆菌中将hyuH2基因表达为麦芽糖结合蛋白（MalE）和内含蛋白-壳多糖结合域（CBD）融合蛋白。 malE-hyuH2的表达导致产生更多的可溶性和活性蛋白。关于温度稳定性，最佳pH和最佳温度，底物和立体特异性，纯化的融合酶表现出与野生型酶相似的性质。 [参考：23]

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