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首页> 外文期刊>Applied Microbiology and Biotechnology >Acetyl-CoA synthetase overexpression in Escherichia coli demonstrates more efficient acetate assimilation and lower acetate accumulation: a potential tool in metabolic engineering
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Acetyl-CoA synthetase overexpression in Escherichia coli demonstrates more efficient acetate assimilation and lower acetate accumulation: a potential tool in metabolic engineering

机译：大肠杆菌中的乙酰辅酶A合成酶过表达证明了更有效的乙酸同化和较低的乙酸积累：代谢工程中的潜在工具

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摘要

The overexpression of acetyl-CoA (CoA) synthetase (ACS) in Escherichia coli showed significant reduction in acetate during glucose fermentation. It also greatly enhanced acetate assimilation when acetate was used as a carbon source. These features are ideal for applications in metabolic engineering. ACS overexpression can be strategically applied to reduce acetate byproduct, recover wasted carbon, and redirect carbon flux toward more favorable pathways. The native acs gene was cloned and overexpressed in E. coli. Studies showed significant effects on acetate production and assimilation in cultures grown in minimal and complex media with glucose or acetate as the carbon source.
机译：乙酰辅酶A（CoA）合成酶（ACS）在大肠杆菌中的过表达表明葡萄糖发酵过程中乙酸盐的显着减少。当乙酸盐用作碳源时，它也大大增强了乙酸盐的同化作用。这些功能非常适合代谢工程中的应用。 ACS过表达可以策略性地应用于减少乙酸盐副产物，回收浪费的碳并将碳通量重定向至更有利的途径。克隆了天然acs基因并在大肠杆菌中过表达。研究表明，在以葡萄糖或乙酸盐为碳源的简单复杂培养基中培养的培养物中，乙酸盐的产生和同化作用具有显着影响。

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