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首页> 外文期刊>Biochimica et biophysica acta. Molecular cell research >Temperature-dependent differences between readily releasable and reserve pool vesicles in chromaffin cells
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Temperature-dependent differences between readily releasable and reserve pool vesicles in chromaffin cells

机译：嗜铬细胞中易释放和储备池囊泡之间的温度依赖性差异

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摘要

Statistical differences between amperometric traces recorded from chromaffin cells using K+ and Ba2+ secretagogues support the assertion that readily releasable pool (RRP) and reserve pool (RP) vesicles can be probed with pool-specific secretagogues. Release from the RRP was evoked by K+ while release from the RP was evoked by Ba2+. Similar temperature-dependent changes in spike area and half-width for both pools suggest that the content of RRP and RP vesicles is similar and packaged in the same way. Differences between the vesicle pools were revealed in the temperature dependence of spike frequency. While the burst spike frequency of the RRP, which is comprised of pre-docked and primed vesicles, increased 2.8% per °C, the RP spike frequency increased 12% per °C. This difference is attributed to a temperature-dependent mobilization of the RP. Furthermore, the RP exhibited more foot events at room temperature than the RRP but this difference was not apparent at 37 °C. This trend suggests that RP vesicle membranes have a compromised surface tension compared to RRP vesicles. Collectively, the changes of release characteristics with temperature reveal distinctions between the RRP and the RP.
机译：使用K +和Ba2 +促分泌素从嗜铬细胞中记录的安培迹线之间的统计差异支持以下主张：可以用特定于池的促分泌素探测易于释放的池（RRP）和储备池（RP）囊泡。从RRP释放由K +引起，而从RP释放由Ba2 +引起。两个池的峰面积和半峰宽度类似的温度依赖性变化表明，RRP和RP囊泡的含量相似且包装方式相同。囊泡池之间的差异显示出尖峰频率对温度的依赖性。虽然由预装填和灌注底液的囊泡构成的RRP的突发峰值频率每°C增加2.8％，但RP峰值频率每°C增加12％。该差异归因于RP的温度依赖性动员。此外，RP在室温下比RRP表现出更多的足部事件，但是这种差异在37°C下并不明显。这种趋势表明，与RRP囊泡相比，RP囊泡膜的表面张力有所降低。总的来说，释放特性随温度的变化揭示了RRP和RP之间的区别。

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