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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Highly Efficient Cationic Ethylphosphatidylcholine siRNA Carrier for GFP Suppression in Modified Breast Cancer Cells.
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Highly Efficient Cationic Ethylphosphatidylcholine siRNA Carrier for GFP Suppression in Modified Breast Cancer Cells.

机译：在修饰的乳腺癌细胞中抑制GFP的高效阳离子乙基磷脂酰胆碱siRNA载体。

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Aim: Cationic ethylphosphatidylcholines (ePCs) were evaluated for the delivery of siRNA in modified breast cancer cells.Dimyristoleoyl-ePC (C14), dioleoyl-ePC (C18), and dilauroyl-ePC (C12) nanoparticles were complexed with siRNA for green fluorescent protein (GFP) suppression in modified MCF-7 breast cancer cells. The kinetics of GFP suppression were followed over the course of 72 hours.C14, which has been previously found to be particularly effective in gene transfection into primary human umbilical artery endothelial cells, was also remarkably effective as siRNA carrier, with an efficacy exceeding that of Lipofectamine RNAiMAX. The C14 toxicity remained comparable to that of RNAiMAX. The efficacy of the other tested cationic ePC formulations was less than that of C14 and RNAiMAX.The cationic lipid C14 is a highly efficient siRNA carrier that could be used for the development of new formulations for siRNA delivery into cancer cells. A valuable advantage of the C14 formulations is the fact that they are simple, and do not require adjuvants or complex preparation procedures.
机译：目的：评估阳离子乙基磷脂酰胆碱（ePCs）在修饰的乳腺癌细胞中的siRNA传递，将二肉豆蔻酰基ePC（C14），二油酰基-ePC（C18）和二月桂酰基-ePC（C12）纳米粒子与siRNA络合以获得绿色荧光蛋白（GFP）修饰的MCF-7乳腺癌细胞中的抑制作用。在72小时的过程中跟踪了GFP的抑制动力学。先前发现C14在将基因转染到人脐静脉内皮细胞中特别有效，它作为siRNA载体也非常有效，其功效超过了C14。脂质体RNAiMAX。 C14毒性仍然与RNAiMAX相当。其他经过测试的阳离子ePC制剂的功效低于C14和RNAiMAX。阳离子脂质C14是高效的siRNA载体，可用于开发用于将siRNA递送至癌细胞的新制剂。 C14制剂的一个重要优点是它们简单，不需要佐剂或复杂的制备程序。

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