Structures of restriction endonuclease HindIII in complex with its cognate DNA and divalent cations

WatanabeN.; TakasakiY.; SatoC.AndoS.TanakaI.

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Structures of restriction endonuclease HindIII in complex with its cognate DNA and divalent cations

机译：限制性内切核酸酶结构HindIII复杂的DNA同源和二价阳离子

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The three-dimensional crystal structures of HindIII bound to its cognate DNA with and without divalent cations were solved at 2.17 and 2.00 ? resolution, respectively. HindIII forms a dimer. The structures showed that HindIII belongs to the EcoRI-like (α-class) subfamily of type II restriction endonucleases. The cognate DNA-complex structures revealed the specific DNA-recognition mechanism of HindIII by which it recognizes the palindromic sequence A/AGCTT. In the Mg2+ ion-soaked structure the DNA was cleaved and two ions were bound at each active site, corresponding to the two-metal-ion mechanism.

机译：三维晶体结构HindIII绑定到其同源基因二价阳离子在2.17和2.00解决吗?分别解决。结构表明,HindIII属于EcoRI-like(α海尔集团)II型的亚科限制内切酶。揭示了特定dna复合结构由它DNA-recognition HindIII机制识别/ AGCTT回文序列。Mg2 + ion-soaked结构DNA裂解和两个离子在每个活性部位,two-metal-ion对应机制。

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《Acta crystallographica.Section D. Biological crystallography》 |2009年第12期|1326-1333|共8页
作者
WatanabeN.; TakasakiY.; SatoC.AndoS.TanakaI.;
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作者单位

Department of Biotechnology and Biomaterial Chemistry, Graduate School of Engineering, Nagoya;

Division of Molecular Life Science, Faculty of Advanced Life Science, Hokkaido University, Japan;

Faculty of Medicine, Saga University, Japan;

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正文语种英语
中图分类化学;
关键词
DNA; DNA Restriction Enzymes; Deoxyribonuclease HindIIIDivalent Cations;

机译：HindIIIDivalent阳离子;

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Structures of restriction endonuclease HindIII in complex with its cognate DNA and divalent cations

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