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首页> 外文期刊>Antimicrobial agents and chemotherapy. >From vanA Enterococcus hirae to vanA Enterococcus faecium: a study of feed supplementation with avoparcin and tylosin in young chickens.
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From vanA Enterococcus hirae to vanA Enterococcus faecium: a study of feed supplementation with avoparcin and tylosin in young chickens.

机译：从平凡肠球菌到粪便肠球菌：在小鸡中补充阿伏霉素和泰乐菌素的饲料的研究。

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Fifteen newborn chickens were isolated in separate cages after 1 month of living together, divided into three groups, and challenged for 5 weeks with seed food which either was supplemented with avoparcin (10 mg/kg of animal food) or tylosin (40 mg/kg) or was nonsupplemented. At 9 weeks of age and after the 5-week challenge, all chickens received nonsupplemented feed for 4 additional weeks. At 4, 9, and 13 weeks of life, feces were collected and inoculated on M-Enterococcus agar plates with and without vancomycin (4 micrograms/ml). vanA-containing Enterococcus hirae was isolated from 11 of 15 chickens before antibiotic challenge, without detection of vancomycin-resistant Enterococcus faecium. At 9 weeks of age and after the 5-week avoparcin challenge, vanA E. hirae strains were no longer detected, but five of five chickens now had vanA E. faecium. At a lower frequency, vanA E. faecium had also displaced vanA E. hirae in both the tylosin group (one of four chickens) and the control group (two of five chickens). One month after avoparcin discontinuation, the number of chickens colonized with vanA E. faecium decreased from five to one. All vanA-containing E. hirae strains detected in the first month of life and most of the vanA-containing E. faecium strains detected in the second month of life showed identical ApaI and SmaI restriction patterns, respectively, when analyzed by pulsed-field gel electrophoresis. All vanA E. hirae isolates transferred glycopeptide and macrolide resistance to Enterococcus faecalis JH2-2 in vitro; the level of glycopeptide resistance was higher in the transconjugants than in the donor E. hirae strains. These data suggest that E. hirae may be a significant source of vanA determinants with the potential of transfer to other enterococcal species from humans or animals.
机译：在一起生活1个月后，将15只新生鸡隔离在单独的笼子中，分为三组，并用种子食物攻击5周，该种子食物补充有阿伏霉素（10 mg / kg动物食物）或泰乐菌素（40 mg / kg） ）或未补充。在9周龄时和5周攻击后，所有鸡都接受了4周以上的非补充饲料。在第4、9和13周的生命中，收集粪便并将其接种在有或没有万古霉素（4微克/毫升）的M-肠球菌琼脂平板上。在抗生素攻击前，从15只鸡中的11只鸡中分离出含有vanA的平肠肠球菌，但未检测到耐万古霉素的粪肠球菌。在9周龄时，在经过5周的阿伏巴霉素攻击后，不再检测到vanA。hirae菌株，但是现在五只鸡中有五只具有粪肠球菌。在较低的频率下，在泰乐菌素组（四只鸡中的一只）和对照组（五只鸡中的两只）中，vanA。faecium粪便也取代了vanA。hirae。停用阿伏帕星一个月后，被vanA粪肠球菌定殖的鸡只数量从五只减少到一只。当通过脉冲场凝胶分析时，在生命的第一个月中检测到的所有含vanA的埃里希氏菌菌株和在生命的第二个月中检测到的大多数含vanA的粪便埃希菌菌株分别显示出相同的ApaI和SmaI限制模式。电泳。所有vanA。hirae分离株均在体外对粪肠球菌JH2-2转移了糖肽和大环内酯抗性;转结合体中糖肽抗性水平高于供体大肠杆菌。这些数据表明，大肠埃希菌可能是vanA决定簇的重要来源，有可能从人或动物转移到其他肠球菌物种。

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