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首页> 外文期刊>Archives of Andrology: An International Journal >Nucleons, I: A model for studying the mechanism of sperm nucleus swelling in vitro.
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Nucleons, I: A model for studying the mechanism of sperm nucleus swelling in vitro.

机译：核子，I：用于研究体外精子核膨胀机制的模型。

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摘要

The nucleon, a highly organized chromatin structure, was studied to learn if its swelling takes place by the action of heparin/GSH, without the participation of any mechanism provided by sperm membranes, subcellular organelles, or other proteins foreign to the sperm nucleus. Sperm suspensions of guinea pigs and rats were incubated with 9 mM DTT and 1% CTAB. The nucleons obtained from washed epididymal spermatozoa appear under a phase-contrast microscope to preserve their original nucleus shape and to completely lack the acrosome, middle piece, and tail. In an electron microscope, nucleon thin sections show a slight nuclear chromatin decompressed from the periphery toward the center. An outstanding result was that the nucleon swelling pattern by heparin/GSH showed the same classic organization into hub-like nuclear bodies joined by a network of chromatin fibers ranging in thickness from 25 to 1.5 nm. Under the conditions of this study there was no need of any membrane or subcellular structure. At stage IV, all the thick fibers disappear, leaving only thin bead fibers on a string. With respect to nuclear swelling there is no doubt that the sperm chromatin is organized in a special form that decides a specific required pattern of unpacking.
机译：研究了核子（一种高度组织的染色质结构），以了解其膨胀是否通过肝素/ GSH的作用发生，而没有精子膜，亚细胞器或精子核外的其他蛋白质提供的任何机制参与。将豚鼠和大鼠的精子悬浮液与9 mM DTT和1％CTAB孵育。从冲洗的附睾精子中获得的核子在相差显微镜下出现，以保留其原始核形状，并完全缺少顶体，中间部分和尾部。在电子显微镜中，核子的薄片显示出轻微的核染色质，从周边向中心减压。出色的结果是，肝素/ GSH的核子膨胀模式显示出相同的经典组织，形成了类似中心分子的核体，并由染色质纤维网络（厚度范围为25至1.5 nm）连接在一起。在这项研究的条件下，不需要任何膜或亚细胞结构。在阶段IV中，所有的粗纤维消失了，只在细绳上留下细的珠子纤维。关于核肿胀，毫无疑问，精子染色质是以一种特殊的形式组织的，它决定了所需的特定拆包模式。

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