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Fiber-optic bundle design for quantitative fluorescence measurement from tissue

机译：光纤束设计，用于组织中定量荧光测量

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摘要

We demonstrate a new design for a fiber-optic bundle to measure fluorescence signals from tissue. In this design, the intensity of the signal is not significantly affected by the medium's absorption and scattering coefficients and hence depends only on the fluorophore's properties. Monte Carlo simulations of light scattering were used for designing and verifying the results obtained. The fiber-optic bundle was tested on tissue-simulating phantoms and compared with a standard nonimaging fiber-optic bundle. The new bundle was composed of 30 individual 100-mu m fibers. Fibers on the end of the bundle that touch the tissue surface were separated from one another by approximately 1 mm. This design permits integration of the signal over several locations while maintaining localized sampling from regions smaller than the average mean free scattering path of the tissue. The bundle was tested by measurement of the fluorescence signals from tissue-simulating solutions containing fluorescent compounds. These studies demonstrate that the new bundle reduces the effect of the intrinsic absorption in the medium, permitting detection of fluorescence that is linearly proportional to the fluorophore concentration. (C) 1998 Optical Society of America OCIS codes: 170.3660, 170.3890, 170.5180, 170.6280, 290.7050, 300.2530. [References: 30]
机译：我们展示了一种用于测量来自组织的荧光信号的光纤束的新设计。在这种设计中，信号的强度不受介质的吸收和散射系数的显着影响，因此仅取决于荧光团的性质。蒙特卡罗模拟的光散射用于设计和验证所获得的结果。在组织模拟体模上测试了光纤束，并将其与标准的非成像光纤束进行了比较。新的纤维束由30根100微米的纤维组成。束末端接触组织表面的纤维彼此分开大约1毫米。这种设计允许在多个位置上整合信号，同时保持来自小于组织平均平均自由散射路径的区域的局部采样。通过测量来自包含荧光化合物的组织模拟溶液的荧光信号来测试束。这些研究表明，新的束减少了介质中固有吸收的影响，从而可以检测与荧光团浓度成线性比例的荧光。 （C）1998年美国光学学会OCIS代码：170.3660、170.3890、170.5180、170.6280、290.7050、300.2530。 [参考：30]

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