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首页> 外文期刊>Archives of microbiology >ORGANOMERCURIAL RESISTANCE DETERMINANTS IN PSEUDOMONAS K-62 ARE PRESENT ON TWO PLASMIDS
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ORGANOMERCURIAL RESISTANCE DETERMINANTS IN PSEUDOMONAS K-62 ARE PRESENT ON TWO PLASMIDS

机译：假单胞菌K-62中的有机物抗药性测定因子存在于两种质粒中

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Pseudomonas strain K-62 was found to contain six plasmids. A mutant derivative cured of the 26-kb plasmid showed a higher sensitivity to mercurials; however, the strain was still able to volatilize them. Loss of the 68-kb plasmid in addition to the 26-kb plasmid abolished the ability of mercury volatilization in this strain and led to a further decrease in the level of mercurial resistance. These results are the first to demonstrate that the organomercurial resistance or Pseudomonas strain K-62 is plasmid-based, and that both the 26- and 68-kb plasmids are required for full expression of the mercurial resistance. Probes specific for the mer genes merA, merB, and merR strongly hybridized with the 26-kb plasmid, but not with the 68-kb plasmid. Two fragments of the 26-kb plasmid that hybridized with the mer genes were cloned and expressed in Escherichia coli. One recombinant plasmid (pMRA17) inducibly encoded a typical broad-spectrum mercurial resistance, whereas the other recombinant plasmid (pMRB01) constitutively conferred hypersensitivity to phenylmercury in the absence of mercuric reductase activity. The results suggest that the two organomercurial lyases in the cells are transcribed from different operator-promoters. [References: 18]
机译：发现假单胞菌菌株K-62含有六个质粒。治愈了26kb质粒的突变体衍生物对汞的敏感性更高;但是，该菌株仍然能够使它们挥发。除26kb质粒外，68kb质粒的缺失消除了该菌株中汞挥发的能力，并导致汞抗性水平进一步降低。这些结果是第一个证明有机汞抗性或假单胞菌菌株K-62是基于质粒的，并且26-kb和68-kb的质粒都需要充分表达汞抗性。对mer基因merA，merB和merR特异的探针与26-kb质粒强烈杂交，但与68-kb质粒没有强杂交。克隆了与mer基因杂交的26 kb质粒的两个片段，并在大肠杆菌中表达。一个重组质粒（pMRA17）诱导编码典型的广谱汞抗性，而另一个重组质粒（pMRB01）在没有汞还原酶活性的情况下组成性地赋予对苯汞超敏性。结果表明，细胞中的两种有机汞裂解酶是从不同的操纵子启动子转录而来的。 [参考：18]

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