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首页> 外文期刊>Archives of microbiology >Isolation of a novel bacterium, Blautia glucerasei sp. nov., hydrolyzing plant glucosylceramide to ceramide.
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Isolation of a novel bacterium, Blautia glucerasei sp. nov., hydrolyzing plant glucosylceramide to ceramide.

机译：新型细菌Blautia glucerasei sp。的分离。十一月，将植物葡萄糖神经酰胺水解为神经酰胺。

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A bacterial strain that is capable of hydrolyzing plant glucosylceramide (GluCer) was newly isolated from dog feces. The novel strain, designated as strain HFTH-1(T), hydrolyzed plant GluCer with a variety of chemical structures, but did not hydrolyze glucosylsphingosine, lactosylceramide, or monosialoganglioside GM(3), indicating that strain HFTH-1(T) produces GluCer-specific glucosylceramidase. Strain HFTH-1(T) was Gram-positive, anaerobic, oval-spore-forming, rod-shaped, lecithinase-negative, and lipase-negative. It fermented a wide variety of carbohydrates and produced mainly acetate, formate, and lactate from glucose. The G + C content of its DNA was 40.7 mol%. The phylogenetic analysis of 16S rRNA sequence revealed that strain HFTH-1(T) is placed in the clostridial rRNA cluster XIVa, with Ruminococcus obeum as the nearest relative. Pairwise comparison revealed approximately 5.0% sequence divergence between strain HFTH-1(T) and the type strain of R. obeum. On the basis of its phenotypic characteristics and phylogenetic divergence, it is proposed that the hitherto unknown rod-shaped bacterial strain HFTH-1(T) (= DSM 22028(T) = NBRC 104932(T)) should be placed in the genus Blautia as a novel species, Blautia glucerasei sp. nov, the only currently known isolate of the species.
机译：从狗粪便中新分离出一种能够水解植物葡糖神经酰胺（GluCer）的细菌菌株。命名为HFTH-1（T）的新菌株水解了具有多种化学结构的植物GluCer，但未水解葡萄糖基鞘氨醇，乳糖基神经酰胺或单唾液酸神经节苷脂GM（3），表明HFTH-1（T）菌株产生了GluCer。特异的葡萄糖基神经酰胺酶。菌株HFTH-1（T）为革兰氏阳性，厌氧，椭圆形孢子形成，杆状，卵磷脂酶阴性和脂肪酶阴性。它发酵各种各样的碳水化合物，并主要从葡萄糖中产生乙酸盐，甲酸盐和乳酸盐。其DNA的G + C含量为40.7mol％。 16S rRNA序列的系统发育分析表明，菌株HFTH-1（T）被放置在梭菌rRNA簇XIVa中，以Ruminococcus obeum为近亲。配对比较显示菌株HFTH-1（T）和R. obeum型菌株之间的序列差异约为5.0％。根据其表型特征和系统发育差异，建议将迄今未知的棒状细菌菌株HFTH-1（T）（= DSM 22028（T）= NBRC 104932（T））放在Blautia属中作为一种新物种，Blautia glucerasei sp。 nov，该物种目前唯一已知的分离株。

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