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Methanobrevibacter filiformis sp. nov., a filamentous methanogen from termite hindguts

机译:丝状甲烷短杆菌十一月,白蚁后肠的丝状产甲烷菌

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A morphologically distinct, filamentous methanogen was isolated from hindguts of the subterranean termite, Reticulitermes flavipes (Kollar) (Rhinotermitidae), wherein it was part of the microbiota colonizing the hindgut wall. Individual filaments of strain RFM-3 were 0.23-0.28 mu m in diameter and usually > 50 mu m in length and aggregated into flocs that were often greater than or equal to 0.1 mm in diameter. Optimal growth of strain RFM-3 was obtained at pH 7.0-7.2 and 30 degrees C with a yeast-extract-supplemented, dithiothreitol-reduced medium in which cells produced stoichiometric amounts of methane from H-2 + CO2. The morphology and gram-positive staining reaction of strain RFM-3, as well as its resistance to cell lysis by various chemical agents and its restriction to H-2 + CO2 as an en ergy source, suggested that it was a member of the Methanobacteriaceae. The nucleotide sequence of the SSU-rRNA-encoding gene of strain RFM-3 confirmed this affiliation and also supported its recognition as a new species of Methanobrevibacter, for which the epithet filiformis is herewith proposed. Although M. filiformis was one of the dominant methanogens in R. flavipes collected from Woods Hole (Mass., USA), cells of similar morphology were not consistently observed in R. flavipes collected from different geographical locations. [References: 11]
机译:从地下白蚁网状黄蚁(Kollar)(Rhinotermitidae)的后肠中分离出形态上不同的丝状产甲烷菌,其中它是定居在后肠壁上的微生物群的一部分。菌株RFM-3的细丝直径为0.23-0.28微米,长度通常> 50微米,并且聚集成絮状物,絮状物的直径通常大于或等于0.1毫米。用补充酵母的,二硫苏糖醇还原的培养基在pH 7.0-7.2和30摄氏度下获得RFM-3菌株的最佳生长,在该培养基中细胞从H-2 + CO2产生化学计量的甲烷。菌株RFM-3的形态和革兰氏阳性染色反应,以及其对各种化学试剂对细胞裂解的抵抗力以及对H-2 + CO2作为能源的限制,表明它是甲烷菌科的一员。 。菌株RFM-3的SSU-rRNA编码基因的核苷酸序列证实了这种隶属关系,并且也支持将其识别为新的甲型短杆菌属,因此建议将其命名为丝状。尽管丝状支原体是从伍兹霍尔(美国马萨诸塞州)收集的黄萎病菌的主要产甲烷菌之一,但在从不同地理位置收集的黄萎病菌中未始终观察到相似形态的细胞。 [参考:11]

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