• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Archives of microbiology >PLASMID RFLP PROFILING AND DNA HOMOLOGY IN THERMUS ISOLATED FROM HOT SPRINGS OF DIFFERENT GEOGRAPHICAL AREAS
【24h】

PLASMID RFLP PROFILING AND DNA HOMOLOGY IN THERMUS ISOLATED FROM HOT SPRINGS OF DIFFERENT GEOGRAPHICAL AREAS

机译：不同地理区域热泉分离的质粒RFLP图谱和DNA同源性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Thirty-four strains belonging to various species of the genus Thermus (T. aquaticus, ''T. thermophilus,'' ''T. brockianus,'' T. scotoductus, and genomic species 2) isolated from hot springs of different geographical areas were examined for plasmid content and restriction fragment length polymorphism (RFLP) of plasmid DNAs. The four strains of the numerical taxonomy cluster E of genomic species 2 did not harbor plasmid DNA. Overall examination of the HindIII-RFLP profiling of plasmid DNA showed considerable variability between and within genomic species, with the exception of presumed clonal isolates. In spite of this heterogeneity, HindIII plasmid digests within a numerical taxonomic cluster gave a subset of restriction fragments of similar or identical length. Strains belonging to genomic species 2 or unclassified isolates from S. Pedro do Sul that harbored plasmid DNA (7 of the 14 strains studied) exhibited strong DNA homology between plasmid regions. No homologous sequences to these plasmid regions were found in chromosomal DNA from strains isolated from S. Pedro do Sul in which no plasmids were detected. The strains belonging to T. scoroductus formed two plasmid DNA homology groups, as estimated by probing with a plasmid fragment that coincided with the two numerical taxonomy clusters proposed previously. Among the other species, homology of plasmid regions was also found between some strains. Strong homology was also found between plasmid regions from some strains of different taxonomic groups, isolated from the same and from different sources, suggesting that these sequences are highly conserved in plasmids present in Thermus. For plasmid-containing strains, results of plasmid RFLP profiling/DNA homology appear promising for the typing of Thermus at the level of biotypes or of individual strains, namely, for monitoring the diversity and frequency of isolates from a particular hot spring. [References: 28]
机译：从不同地理区域的温泉中分离出来的34种菌株分别属于Thermus菌种（T. aquaticus，``T。thermophilus''，``T。brockianus''，T。scotoductus和基因组2）。检查质粒含量和质粒DNA的限制性片段长度多态性（RFLP）。基因组物种2的数字分类学簇E的四个菌株没有携带质粒DNA。对质粒DNA的HindIII-RFLP分析的总体检查显示，基因组物种之间和内部存在相当大的变异性，但推测的克隆分离株除外。尽管存在这种异质性，但在数字分类学簇内的HindIII质粒消化却产生了相似或相同长度的限制性片段的子集。属于基因组种2或来自S. Pedro do Sul的未分类分离株的菌株含有质粒DNA（研究的14个菌株中的7个）在质粒区域之间表现出很强的DNA同源性。在从没有检测到质粒的S.Pedro do Sul分离的菌株的染色体DNA中未发现与这些质粒区域的同源序列。如通过用与先前提出的两个数字分类学簇相符的质粒片段探测所估计的那样，属于T. scoroductus的菌株形成了两个质粒DNA同源性组。在其他物种中，在某些菌株之间也发现了质粒区域的同源性。在来自不同分类组的一些菌株的质粒区域之间也发现了强同源性，这些菌株从相同和不同来源分离，表明这些序列在Thermus中存在的质粒中高度保守。对于含质粒的菌株，质粒RFLP分析/ DNA同源性的结果看来有望在生物型或单个菌株的水平上对Thermus进行分型，即用于监测来自特定温泉的分离物的多样性和频率。 [参考：28]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号