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首页> 外文期刊>Archives of microbiology >CLONING, NUCLEOTIDE SEQUENCE, AND EXPRESSION OF THE GENE ENCODING A NOVEL DIOXYGENASE INVOLVED IN METABOLISM OF CARBOXYDIPHENYL ETHERS IN PSEUDOMONAS PSEUDOALCALIGENES POB310
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CLONING, NUCLEOTIDE SEQUENCE, AND EXPRESSION OF THE GENE ENCODING A NOVEL DIOXYGENASE INVOLVED IN METABOLISM OF CARBOXYDIPHENYL ETHERS IN PSEUDOMONAS PSEUDOALCALIGENES POB310

机译：假单胞菌假单胞菌POB310中涉及羧二苯醚代谢的新型双氧合酶基因的克隆，核苷酸序列及表达

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Pseudomonas pseudoalcaligenes strain POB310 degrades 3- and 4-carboxydiphenyl ether. The initial reaction involves an angular dioxygenation yielding an unstable hemiacetal that spontaneously decays to phenol and protocatechuate. We cloned a DNA fragment containing the gene encoding the initial dioxygenase from an unstable, self-transmissible plasmid. Sequence analysis revealed two open reading frames encoding proteins with putative molecular masses of 46.3 and 33.6 kDa. The deduced amino acid sequences showed homologies to oxygenase and reductase subunits of aromatic ring-activating dioxygenases, and contained regions identical to consensus sequences that bind chloroplast-like and Rieske-type [2Fe2S] clusters, suggesting that the initial dioxygenase is a class IA aromatic ring-activating dioxygenase system. Initial dioxygenase activity was induced in bacteria grown in M9 minimal medium containing 3- or 4-carboxydiphenyl ether or phenol as carbon source, indicating that the regulation is dependent on the phenol pathway. The maximal specific activity was measured at the beginning of the exponential growth phase. [References: 40]
机译：假单胞菌假产碱菌POB310降解3-和4-羧基二苯醚。最初的反应涉及角双加氧，产生不稳定的半缩醛，该半缩醛自发地分解为苯酚和原儿茶酸盐。我们从一个不稳定的，自我传播的质粒中克隆了一个DNA片段，该片段包含编码初始双加氧酶的基因。序列分析揭示了两个开放阅读框，它们编码蛋白质的推定分子质量为46.3和33.6 kDa。推导的氨基酸序列显示出与芳香环活化双加氧酶的加氧酶和还原酶亚基的同源性，并包含与结合叶绿体样和Rieske型[2Fe2S]簇的共有序列相同的区域，这表明最初的双加氧酶是IA类芳香族环活化双加氧酶系统。在含有3-或4-羧基二苯醚或苯酚作为碳源的M9基本培养基中生长的细菌中诱导了最初的双加氧酶活性，表明该调节取决于苯酚途径。在指数生长期开始时测量最大比活。 [参考：40]

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