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首页> 外文期刊>Archives of microbiology >EXTRACELLULAR IRON REDUCTASE ACTIVITY PRODUCED BY LISTERIA MONOCYTOGENES
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EXTRACELLULAR IRON REDUCTASE ACTIVITY PRODUCED BY LISTERIA MONOCYTOGENES

机译：荔枝单胞菌产生的胞外铁还原酶活性

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Little is known about how pathogenic microorganisms that do not produce low-molecular-weight iron-chelating agents, termed siderophores, acquire iron from their environment. We have identified an extracellular enzyme produced by Listeria monocytogenes that can mobilize iron from a variety of iron-chelate complexes via reduction of the metal. The iron reductase requires Mg2+, flavin mononucleotide (FMN), and reduced nicotinamide adenine dinucleotide (NADH) for activity. Saturation kinetics were found when initial velocity studies of iron reduction were carried out as a function of variable FMN concentrations in the presence of 100 mu M NADH and 10 mM Mg2+. Hyperbolic kinetics were also found when these studies were repeated as a function of variable NADH concentrations along with 20 mu M FMN and 10 mM Mg2+. This process of extracellular reduction, in all likelihood, could be involved in the mobilization of iron from soils and aqueous environments and from host tissues in pathogenic processes, This is the first report of the extracellular enzymic reduction of iron by microorganisms. [References: 25]
机译：对于不产生低分子量铁螯合剂（称为铁载体）的病原微生物如何从其环境中获取铁知之甚少。我们已经确定了单核细胞增生李斯特菌产生的一种细胞外酶，该酶可以通过还原金属从多种铁螯合物中调集铁。铁还原酶需要Mg2 +，黄素单核苷酸（FMN）和还原的烟酰胺腺嘌呤二核苷酸（NADH）才能发挥活性。当存在100μM NADH和10 mM Mg2 +时，进行铁还原的初始速度研究作为可变FMN浓度的函数时，发现了饱和动力学。当重复这些研究时，还发现了双曲动力学与可变的NADH浓度以及20μM FMN和10 mM Mg2 +的关系。这种细胞外还原的过程很可能与病原过程中土壤和水环境以及宿主组织中铁的迁移有关。这是微生物对铁进行细胞外酶促还原的首次报道。 [参考：25]

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