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Sphingomonas panaciterrae sp nov., a plant growth-promoting bacterium isolated from soil of a ginseng field

机译:Sphingomonas panaciterrae sp nov。,一种从人参田土壤中分离出来的植物生长细菌

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Strain DCY91(T), a Gram-stain-negative, rod-shaped, aerobic, non-motile bacterium, was isolated from soil of ginseng field in Gyeonggi province, South Korea. Strain DCY91(T) shared the highest 16S rRNA gene sequence similarity with Sphingomonas mucosissima DSM 17494(T) (98.55 %), Sphingomonas dokdonensis KACC 17420(T) (98.11 %) and Sphingomonas xinjiangensis DSM 26736(T) (96.68 %). The strain DCY91(T) was found to able to grow best in trypticase soy agar at 28 A degrees C, at pH 7 and at 0.5 % NaCl. Ubiquinone 10 was identified as the isoprenoid quinone. The major polar lipids were identified as sphingoglycolipid, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The major fatty acids of strain DCY91(T) were identified as unsaturated C-18:1 omega 7c and saturated C-16:0. The major polyamine content was sym-homospermidine. The DNA G + C content was determined to be 65.8 mol% (HPLC). After 6 days of incubation, strain DCY91(T) produced 9.64 +/- A 1.73 and 33.73 +/- A 4.66 A mu g/ml indole-3-acetic acid, using media without l-tryptophan and supplemented with l-tryptophan, respectively. Strain DCY91(T) was also weakly solubilized phosphate and produced siderophores. On the basis of the phenotypic characteristics, genotypic analysis and chemotaxonomic characteristics, strain DCY91(T) is considered to represent a novel species of the genus Sphingomonas, for which the name Sphingomonas panaciterrae sp. nov. is proposed. The type strain is DCY91(T) (=KCTC 42346(T) =JCM 30807(T)).
机译:从韩国京畿道的人参田土壤中分离出菌株DCY91(T),它是革兰氏阴性,杆状,好氧,不运动的细菌。菌株DCY91(T)与黏膜鞘氨醇单胞菌DSM 17494(T)(98.55%),dphdonnas dokdonensis KACC 17420(T)(98.11%)和新疆鞘氨醇单胞菌DSM 26736(T)共享最高的16S rRNA基因序列相似性。(96.68%)。发现该菌株DCY91(T)在胰蛋白酶解离的大豆琼脂中在28 A的温度,pH 7和0.5%NaCl的条件下生长最佳。泛醌10被确定为类异戊二烯醌。主要的极性脂质被鉴定为鞘糖脂,二磷脂酰甘油,磷脂酰乙醇胺,磷脂酰甘油和磷脂酰胆碱。 DCY91(T)菌株的主要脂肪酸被鉴定为不饱和C-18:1ω7c和饱和C-16:0。主要的多胺含量是同高嘧啶。 DNA G + C含量经测定为65.8 mol%(HPLC)。孵育6天后,菌株DCY91(T)使用不含l-色氨酸并补充了l-色氨酸的培养基,产生了9.64 +/- A 1.73和33.73 +/- A 4.66 Aμg / ml吲哚-3-乙酸,分别。菌株DCY91(T)也是弱溶解的磷酸盐,并产生铁载体。根据表型特征,基因型分析和化学分类学特征,菌株DCY91(T)被认为代表了鞘氨醇单胞菌属的一个新物种,其名称为鞘氨醇单胞菌。十一月被提议。类型应变为DCY91(T)(= KCTC 42346(T)= JCM 30807(T))。

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