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Variation in swimming speed of Escherichia coli in response to attractant

机译:大肠杆菌对引诱剂的游泳速度变化

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It is well known that Escherichia coli executes chemotactic motion in response to chemical cues by modulating the flagellar motor bias alone. However, previous studies have reported the possibility of variation in run speed in the presence of attractants although it is unclear whether bacteria can deliberately modulate their swimming speeds in response to environmental cues or if the motor speeds are hardwired. By studying the detailed motion of cells in a uniform concentration of glucose and its non-metabolizable analogue, we show that changing concentrations may be accompanied by variation in the swimming speed. For a fixed run duration, cells exposed to the attractants achieved a higher peak-swimming speed after a tumble compared with that in plain motility buffer. Our experiments using the mutant strain lacking the Trg sensor show no change in swimming speed with varying concentrations of the non-metabolizable analogue, suggesting that sensing may play a role in the observed variation of swimming speed.
机译:众所周知,大肠杆菌通过单独调节鞭毛运动偏倚来响应化学提示而执行趋化运动。然而,先前的研究报道了在存在引诱剂的情况下运行速度可能发生变化的可能性,尽管目前尚不清楚细菌是否可以根据环境提示故意调节其游泳速度,或者电机速度是否采用硬接线。通过研究均匀浓度的葡萄糖及其不可代谢类似物中细胞的详细运动,我们发现浓度变化可能伴随着游泳速度的变化。在固定的运行时间内,与纯运动缓冲液相比,暴露于引诱剂的细胞在翻滚后达到了更高的峰游速度。我们使用缺少Trg传感器的突变菌株进行的实验表明,随着不可代谢类似物浓度的变化,游泳速度也没有变化,这表明传感可能在观察到的游泳速度变化中起作用。

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