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首页> 外文期刊>Archives of Toxicology >New methods for determination of 2-butoxyethanol, butoxyacetaldehyde and butoxyacetic acid in aqueous systems, with special reference to cell culture conditions.
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New methods for determination of 2-butoxyethanol, butoxyacetaldehyde and butoxyacetic acid in aqueous systems, with special reference to cell culture conditions.

机译：测定水性体系中2-丁氧基乙醇，丁氧基乙醛和丁氧基乙酸的新方法，尤其要参考细胞培养条件。

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摘要

Ethylene glycol ethers, especially 2-ethoxyethanol and 2-butoxyethanol (BE) are frequently used in industry and household as solvents and detergents because of their excellent hydrophilic and lipophilic properties. BE and its oxidation products, butoxyacetaldehyde (BAL) and butoxyacetic acid (BAA), are mainly associated with haemolytic toxicity. No method to determine BAL in aqueous systems (e.g. urine or blood) has been published up to now. BAL was synthesized by dehydration of BE and identified by gas chromatography-mass spectrometry. For determination of BAL and BE with head space-capillary gas chromatography, water and HCl or sodium dihydrogen phosphate were added to the sample. No further extraction or derivatization were necessary. For BAA determination after adding HCl and sodium dihydrogen phosphate the samples were extracted with ethyl acetate and derivatized with 2,2,2-trichloroethanol/HCl. The analytical methods presented here are reliable, sensitive and rapid. The new methods were developed for mammalian cell culture systems, because such in vitro systems are especially useful for metabolic studies and have the advantage of choosing species and organ specificity. In the cell culture experiments presented here it was demonstrated that Opossum kidney cells are able to metabolize BAL to BAA within 24 h. After this interval, in the cells neither BAL nor BAA were accumulated, whereas BAA was found in the cell culture media.
机译：乙二醇醚，尤其是2-乙氧基乙醇和2-丁氧基乙醇（BE），由于其优异的亲水性和亲脂性，经常在工业和家庭中用作溶剂和清洁剂。 BE及其氧化产物丁氧基乙醛（BAL）和丁氧基乙酸（BAA）主要与溶血毒性有关。迄今为止，尚未公开测定水性系统（例如尿液或血液）中BAL的方法。 BAL通过BE的脱水合成，并通过气相色谱-质谱法鉴定。为了用顶空毛细管气相色谱法测定BAL和BE，将水和HCl或磷酸二氢钠添加到样品中。无需进一步提取或衍生化。为了在加入HCl和磷酸二氢钠后进行BAA测定，将样品用乙酸乙酯萃取，并用2,2,2-三氯乙醇/ HCl衍生化。此处介绍的分析方法可靠，灵敏且快速。为哺乳动物细胞培养系统开发了新方法，因为这种体外系统对代谢研究特别有用，并且具有选择物种和器官特异性的优势。在此处介绍的细胞培养实验中，证明负鼠肾细胞能够在24小时内将BAL代谢为BAA。在此间隔之后，在细胞中既没有BAL也没有BAA积聚，而在细胞培养基中发现了BAA。

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