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首页> 外文期刊>Breeding science >Integrated reference genetic linkage maps of pear based on SSR and AFLP markers.
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Integrated reference genetic linkage maps of pear based on SSR and AFLP markers.

机译：基于SSR和AFLP标记的梨的综合参考遗传连锁图谱。

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Integrated genetic linkage maps of the European pear (Pyrus communis L.) cultivars 'Bartlett' and 'La France' were constructed based on SSR markers developed from pear and apple, AFLP and other markers. The map of 'Bartlett', which was constructed using an F< sub>1 population derived from a cross between 'Bartlett' and 'Housui', consisted of 447 loci, including 58 loci revealed by pear SSR markers (hereafter referred to as "pear SSR loci"), 60 by apple SSR markers (hereafter referred to as "apple SSR loci") and 322 by AFLP markers (hereafter referred to as "AFLP loci"). This map covered 17 linkage groups over a total length of 1,000 cM with an average distance of 2.3 cM between markers. Another genetic linkage map of 'La France' was constructed using F< sub>1 individuals obtained from a cross between 'Shinsei' and '282-12' ('Housui' x 'La France'). The map of 'La France' contained 414 loci, including 66 pear SSR loci, 68 apple SSR loci and 279 AFLP loci on 17 linkage groups encompassing a genetic distance of 1,156 cM. Using 97 common SSR markers, these two maps were well aligned together for all the 17 linkage groups, which corresponded to the basic chromosome number (n=17). The positions of 66 SSR markers originating from apple were successfully determined in pear maps and showed a co-linearity with the saturated reference map of apple. Since the high-density genetic linkage maps of pear constructed in the present study covered almost the entire genome, they should be considered as pear reference maps. These pear reference maps may enable to identify the location of genes of interest and QTLs (quantitative trait loci), and to analyze the syntenic genome structure with other Maloideae species.
机译：欧洲梨（Pyrus communis L.）品种“ Bartlett”和“ La France”的综合遗传连锁图谱是基于梨和苹果，AFLP和其他标记开发的SSR标记构建的。 “ Bartlett”图谱是使用“ Bartlett”和“ Housui”之间的杂交衍生的F 1 种群构建的，由447个基因座组成，其中包括梨SSR标记揭示的58个基因座（以下简称“苹果梨SSR标记称为“梨SSR基因座”，60个苹果SSR标记（以下称为“苹果SSR基因座”）和AFLP标记322（以下称为“ AFLP基因座”）。该图涵盖了17个连锁群，总长度为1,000 cM，标记之间的平均距离为2.3 cM。使用从“ Shinsei”和“ 282-12”（“ Housui” x“ La France”）的杂交中获得的F 1 个人构建了“ La France”的另一条遗传连锁图谱。 “法国”地图包含414个基因座，包括66个梨SSR基因座，68个苹果SSR基因座和279个AFLP基因座，位于17个连锁群上，遗传距离为1,156 cM。使用97个常见的SSR标记，对于所有17个连锁组，这两个图谱都很好地对齐在一起，这对应于基本染色体数（n = 17）。在梨图中成功确定了来自苹果的66个SSR标记的位置，并与苹果的饱和参考图显示了共线性。由于本研究中构建的梨的高密度遗传连锁图谱几乎涵盖了整个基因组，因此应将其视为梨参考图谱。这些梨参考图可能使能够识别感兴趣的基因和QTL（定量性状基因座）的位置，并分析与其他Maloideae物种的同义基因组结构。

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